Cervical ossification of the posterior longitudinal ligament (cOPLL) is one of the major causes of myelopathy. However, the mechanism underlying remains elusive. In the present study, using MILLIPLEX magnetic bead panel, we investigated four serum hormones and six serum cytokines in cOPLL patients and healthy subjects. The results showed that tumor necrosis factore-α (TNF-α) were significantly increased, and DDK-1 was significantly decreased in the serum from male and female cOPLL patients compared with those from healthy controls, respectively. Osteopontin (OPN) and fibroblast growth factor-23 (FGF-23) were significantly increased in male cOPLL patients compared with that in healthy male controls. Further analysis showed that FGF-23 and OPN significantly increased, dickkopf-1 (DKK-1) decreased in the extensive cOPLL group. In addition, a significant positive correlation between the OPN and FGF-23 was observed in male cOPLL patients. The results are useful for understanding the mechanism underlying cOPLL.

颈椎后纵韧带骨化（cOPLL）是脊髓病的主要原因之一。但是，潜在的机制仍然难以捉摸。在本研究中，我们使用MILLIPLEX磁珠板对cOPLL患者和健康受试者中的四种血清激素和六种血清细胞因子进行了研究。结果表明，与健康对照组相比，男性和女性cOPLL患者血清中的肿瘤坏死因子-α（TNF-α）显着增加，而DDK-1显着降低。男性cOPLL患者的骨桥蛋白（OPN）和成纤维细胞生长因子23（FGF-23）显着高于健康男性对照组。进一步的分析表明，在广泛的cOPLL组中，FGF-23和OPN显着增加，而dickkopf-1（DKK-1）减少。此外，在男性cOPLL患者中观察到OPN和FGF-23之间的显着正相关。结果对于理解cOPLL的基本机制很有用。