Objective: The objective of the present work was to investigate a possible mechanism of NF-κB signaling pathway and autophagy in the regulation of osteoblast differentiation, and provide experimental basis for the study of tooth eruption disorder.

Methods: Mouse osteoblast-like (MC3T3-E1) cells were inoculated with a cell density of 70%. According to the grouping experimental design, Western blot and monodansylcadaverine (MDC) detection were conducted after dosing for 24 h. The cells were divided into the following five groups: blank control group; 6.25 µg/mL SN50 group; 12.5 µg/mL SN50 group; 25 µg/mL SN50 group and 50 µg/mL SN50 group.

Results: Western blot analysis revealed that the expression of LC3 protein was present in the blank control group; 6.25 µg/mL SN50 group; 12.5 µg/mL SN50 group and 50 µg/mL SN50 group, with no significant differences among these groups. However, the expression of LC3 protein was significantly lower in the 25 µg/mL SN50 group. MDC detection showed that, in the blank control group; 6.25 µg/mL SN50 group; 12.5 µg/mL SN50 group and 50 µg/mL SN50 group, there was obvious green fluorescence in the cytoplasm of the osteoblasts. However, in the 25 µg/mL SN50 group, it was found that there were significantly fewer green fluorescent particles.

Conclusion: The osteoblast itself had a strong function of autophagy. The appropriate concentration of SN50 in blocking the NF-κB pathway of the osteoblast was associated with the obvious inhibition of autophagy. However, the relationship between NF-κB signaling pathway and autophagy in the process of tooth eruption requires further study.

目的：本研究旨在探讨NF-κB信号通路和自噬调控成骨细胞分化的可能机制，为研究牙萌出障碍提供实验依据。

方法：接种小鼠成骨细胞样（MC3T3-E1）细胞，细胞密度为70％。根据分组实验设计，给药24小时后进行Western印迹和单丹磺酰尸胺（MDC）检测。将细胞分为以下五组：空白对照组；和对照组。SN50组为6.25 µg / mL；SN50组12.5 µg / mL；25 µg / mL SN50组和50 µg / mL SN50组。

结果： Western blot分析显示空白对照组中存在LC3蛋白的表达。SN50组为6.25 µg / mL；SN50组为12.5 µg / mL，SN50组为50 µg / mL，两组之间无显着差异。但是，在25 µg / mL SN50组中，LC3蛋白的表达明显降低。MDC检测显示，空白对照组；SN50组为6.25 µg / mL；12.5μg/ mL SN50组和50μg/ mL SN50组，成骨细胞的细胞质中有明显的绿色荧光。但是，在25 µg / mL SN50组中，发现绿色荧光颗粒明显减少。

结论：成骨细胞本身具有很强的自噬功能。适当浓度的SN50阻断成骨细胞的NF-κB通路与自噬的明显抑制有关。但是，在萌发过程中NF-κB信号通路与自噬之间的关系尚需进一步研究。