It has been reported that the receptor-like cytoplasmic kinases (RLCKs) regulate many biological processes in plants, but only a few members have been functionally characterized. Here, we isolated a rice gene encoding AtRRK1 homology protein kinase, OsRRK1, which belongs to the RLCK VI subfamily. OsRRK1 transcript accumulated in many tissues at low to moderate levels and at high levels in leaves. Overexpression of OsRRK1 (OE-OsRRK1) caused adaxial rolling and erect morphology of rice leaves. In the rolled leaves of OE-OsRRK1 plants, both the number and the size of the bulliform cells are decreased compared to the wild-type (WT) plants. Moreover, the height, tiller number, and seed setting rate were reduced in OE-OsRRK1 plants. In addition, the brown planthopper (BPH), a devastating pest of rice, preferred to settle on WT plants than on the OE-OsRRK1 plants in a two-host choice test, indicating that OE-OsRRK1 conferred an antixenosis resistance to BPH. The analysis of transcriptome sequencing demonstrated that several receptor kinases and transcription factors were differentially expressed in OE-OsRRK1 plants and WT plants. These results indicated that OsRRK1 may play multiple roles in the development and defense of rice, which may facilitate the breeding of novel rice varieties.

中文翻译：

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OsRRK1的过表达改变了水稻的叶片形态和对昆虫的防御能力。

据报道，受体样细胞质激酶（RLCKs）调节植物中的许多生物学过程，但只有少数成员具有功能特征。在这里，我们分离了一个水稻基因，该基因编码AtRRK1同源蛋白激酶OsRRK1，该基因属于RLCK VI亚家族。OsRRK1转录本在叶片中以低至中等水平和高水平积累在许多组织中。OsRRK1（OE-OsRRK1）的过度表达导致水稻叶片的近轴滚动和直立形态。与野生型（WT）植物相比，在OE-OsRRK1植物的卷叶中，疱状细胞的数量和大小均减少。此外，OE-OsRRK1植物的株高，分number数和结实率均降低。此外，稻飞虱（BPH）是水稻的毁灭性害虫，在两宿主选择试验中，与在OE-OsRRK1植物上定居相比，更倾向于在WT植物上定居，这表明OE-OsRRK1赋予了BPH抗排异性抗性。转录组测序分析表明，几种受体激酶和转录因子在OE-OsRRK1植物和WT植物中差异表达。这些结果表明OsRRK1可能在水稻的发育和防御中发挥多种作用，这可能有助于水稻新品种的选育。