Bioactivity of cod (Gadus morhua) and chicken (Gallus domesticus) protein hydrolysates before and after in vitro gastrointestinal (GI) digestion was investigated using yeast Saccharomyces cerevisiae as a model organism. Both hydrolysates were exposed to in vitro GI digestion prior to cellular exposure to simulate digestion conditions in the human body and therefore investigate the role of modulations in the GI tract on the cell response. The effect of digested and undigested hydrolysates on intracellular oxidation, cellular metabolic energy and proteome level was investigated. No difference in the effect on intracellular oxidation activity was obtained between cod and chicken hydrolysates, while higher affect on intracellular oxidation was provided by digested hydrolysates, with relative values of intracellular oxidation of cod of (70.2±0.8) and chicken of (74.5±1.4) % than by undigested ones, where values of cod and chicken were (95.5±1.2) and (90.5±0.7) %, respectively. Neither species nor digestion had any effect on cellular metabolic energy. At proteome level, digested hydrolysates gave again significantly stronger responses than undigested counterparts; cod peptides here also gave somewhat stronger response than chicken peptides. The knowledge of the action of food protein hydrolysates and their digests within live cells, also at proteome level, is important for further validation of their activity in higher eukaryotes to develop new functional food ingredients, such as in this case chicken and cod muscle-derived peptides.

中文翻译：

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鳕鱼和鸡肉蛋白水解物在体外胃肠消化之前和之后的生物活性。

使用酵母酿酒酵母（Saccharomyces cerevisiae）作为模型生物，研究了体外胃肠（GI）消化之前和之后的鳕鱼（Gadus morhua）和鸡肉（Galus domesticus）蛋白水解物的生物活性。两种水解物均暴露于体外在暴露于细胞之前进行胃肠消化以模拟人体中的消化条件，因此研究了胃肠道中的调节作用对细胞反应的作用。研究了消化和未消化的水解产物对细胞内氧化，细胞代谢能和蛋白质组水平的影响。鳕鱼和鸡肉的水解产物对细胞内氧化活性的影响没有差异，而消化的水解产物对细胞内氧化的影响更大，鳕鱼和鸡肉的细胞内氧化的相对值为（70.2±0.8）。 ）％，而未消化的鳕鱼和鸡的价值分别为（95.5±1.2）％和（90.5±0.7）％。物种和消化都对细胞代谢能量没有任何影响。在蛋白质组水平上 消化后的水解产物比未消化的对应产物显着增强反应；鳕鱼肽的反应也比鸡肉肽强。了解食物蛋白水解物及其在活细胞内在蛋白质组水平上的消化作用的知识，对于进一步验证其在高等真核生物中的活性以开发新的功能性食物成分（例如在本例中是鸡和鳕鱼肌肉来源）的作用非常重要。肽。