Serum from Kemp's ridley and loggerhead sea turtles was collected in summers of 2011, 2012, and 2013. Serum IgY recognition of lysate proteins from nine bacteria species and whole bacteria-specific IgY titers to these pathogens were quantified. Serum and purified IgY recognized proteins of all bacteria, with protein recognition in some species more pronounced than others. Circulating IgY titers against V. vulnificus, V. anguillarum, E. rhusiopathiae, and B. vesicularis changed over the years in Kemp's ridley turtles, while titers IgY against V. vulnificus, E. coli, V. parahaemolyticus, B. vesicularis, and M. marinum were different in loggerhead turtles. Serum lysozyme activity was constant for loggerhead turtles over the 3 years, while activity in Kemp's ridleys were lower in 2012 than 2013. Blood PCV, glucose levels, and serum protein levels were comparable to healthy turtles in previous studies, therefore, this study provides baseline information on antibody responses in healthy, wild turtles. Received 25 May 2017 accepted 09 Oct 2017 revised 15 Sep 2017.

中文翻译：

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对来自美国东南部的蠵龟、Caretta caretta 和 Kemp'sridley、Lepidochelys kempii 海龟中选择海洋细菌的体液免疫反应

在 2011 年、2012 年和 2013 年夏天收集了肯普氏海龟和红海龟的血清。对来自九种细菌的裂解物蛋白质的血清 IgY 识别和对这些病原体的全细菌特异性 IgY 滴度进行了量化。血清和纯化的 IgY 可识别所有细菌的蛋白质，某些物种的蛋白质识别比其他物种更明显。多年来，Kemp'sridley 龟的循环 IgY 滴度针对 V. vulnificus、V. anguillarum、E. rhusiopathiae 和 B. vesicularis 发生了变化，而针对 V. vulnificus、E.coli、V. parahaemolyticus、B. vesicularis 和 B. vesicularis 的 IgY 滴度则发生了变化。 M. marinum 在红海龟中是不同的。过去 3 年中，红海龟的血清溶菌酶活性保持不变，而 2012 年 Kemp'sridleys 的活性低于 2013 年。血液 PCV、葡萄糖水平、和血清蛋白水平与之前研究中的健康海龟相当，因此，本研究提供了健康野生海龟抗体反应的基线信息。2017 年 5 月 25 日接收，2017 年 10 月 9 日接受，2017 年 9 月 15 日修订。