Loss-of-function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, Random Sequence Labels (RSLs) are incorporated into the guide library, which act as unique molecular identifiers (UMIs) to allow massively parallel lineage tracing and lineage dropout screening. RSLs greatly improve the reproducibility of results by increasing both the precision and the accuracy of screens. They reduce the number of cells needed to reach a set statistical power, or allow a more robust screen using the same number of cells.

中文翻译：

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使用独特的分子标识符进行CRISPR / Cas9筛选。

利用汇集的慢病毒指南文库通过CRISPR / Cas9基因敲除进行功能丧失筛查是一种广泛适用的方法，用于系统识别有助于多种细胞表型的基因。在这里，随机序列标签（RSL）被合并到指南库中，用作唯一的分子标识符（UMI），以允许大规模平行的谱系追踪和谱系缺失筛选。RSL通过提高屏幕的准确性和准确性极大地提高了结果的可重复性。它们减少了达到设定的统计功效所需的像元数量，或者允许使用相同数量的像元进行更强大的筛选。