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Antihemolytic and antioxidant properties of pearl powder against 2,2′-azobis(2-amidinopropane) dihydrochloride-induced hemolysis and oxidative damage to erythrocyte membrane lipids and proteins
Journal of Food and Drug Analysis ( IF 2.6 ) Pub Date : 2017-10-01 , DOI: 10.1016/j.jfda.2016.10.007 Hsin-Ling Yang , Mallikarjuna Korivi , Ming-Kuem Lin , Hebron Chun-Wei Chang , Chi-Rei Wu , Meng-Shiou Lee , William Tzu-Liang Chen , You-Cheng Hseu
Journal of Food and Drug Analysis ( IF 2.6 ) Pub Date : 2017-10-01 , DOI: 10.1016/j.jfda.2016.10.007 Hsin-Ling Yang , Mallikarjuna Korivi , Ming-Kuem Lin , Hebron Chun-Wei Chang , Chi-Rei Wu , Meng-Shiou Lee , William Tzu-Liang Chen , You-Cheng Hseu
Pearl powder, a well-known traditional mineral medicine, is reported to be used for well-being and to treat several diseases from centuries in Taiwan and China. We investigated the in vitro antihemolytic and antioxidant properties of pearl powder that could protect erythrocytes against 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative damage to membrane proteins/lipids. Human erythrocytes were incubated with different concentrations of pearl powder (50-200 μg/mL) for 30 minutes and then exposed to AAPH for 2-6 hours. We found that AAPH alone time dependently increased the oxidative hemolysis of erythrocytes, while pearl powder pretreatment substantially inhibited the hemolysis in a concentration-/time-dependent manner. AAPH-induced oxidative damage to erythrocyte membrane lipids was evidenced by the elevated malondialdehyde (MDA) levels. However, pearl powder remarkably inhibited the malondialdehyde formation, and the 200 μg/mL concentration showed almost similar malondialdehyde values to the control. Furthermore, pearl powder suppressed the AAPH-induced high-molecular-weight protein formation and concomitantly increased the low-molecular-weight proteins in erythrocytes. Antioxidant potential that was measured as superoxide dismutase activity and glutathione content was significantly dropped by AAPH incubation, which suggests the vulnerability of erythrocytes to AAPH-induced oxidative stress. Noteworthy, erythrocytes pretreated with pearl powder showed restored superoxide dismutase activity and glutathione levels against AAPH-induced loss. Our findings conclude that pearl powder attenuate free radical-induced hemolysis and oxidative damage to erythrocyte membrane lipids/proteins. The potent antioxidant property of pearl powder may offer protection from free radical-related diseases.
更新日期：2017-10-01
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