Infections of very young children or immunocompromised people with Salmonella of higher subspecies are a well-known phenomenon often associated with contact to cold-blooded animals. We describe the molecular characterization of three S. enterica subsp. diarizonae strains, isolated consecutively over a period of several months from a hospital patient suffering from diarrhea and sepsis with fatal outcome. With the initial isolate the first complete genome sequence of a member of subsp. diarizonae is provided and based on this reference we revealed the genomic differences between the three isolates by use of next-generation sequencing and confirmed by phenotypical tests. Genome comparisons revealed mutations within gpt, hfq and purK in the first isolate as a sign of clonal variation rather than host-directed evolution. Furthermore, our work demonstrates that S. enterica subsp. diarizonae possess, besides a conserved set of known Salmonella Pathogenicity Islands, a variable portfolio of additional genomic islands of unknown function.

中文翻译：

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三个连续的沙门氏菌分离株的比较全基因组分析。

高龄亚种沙门氏菌感染年幼的儿童或免疫力低下的人是一种众所周知的现象，通常与接触冷血动物有关。我们描述了三个肠球菌亚种的分子表征。几个月来连续从患有腹泻和败血症并有致命后果的住院患者中连续分离出双歧杆菌。对于最初的分离物，亚种成员的第一个完整的基因组序列。提供了二氮杂细菌，并基于该参考文献，我们利用下一代测序揭示了这三种分离物之间的基因组差异，并通过表型测试得到了证实。基因组比较显示第一个分离株中gpt，hfq和purK内的突变是克隆变异而不是宿主定向进化的标志。此外，我们的工作证明了肠炎沙门氏菌亚种。除了一组已知的沙门氏菌致病性孤岛的保守集外，Diarizonae还拥有功能未知的其他基因组孤岛的可变组合。