Soil biota represents a major component of the earth's biodiversity and for over 200 years, the microscopy approach was the only way to explore it. In the last decade, the DNA-based technique has been adopted in soil ecology. Due to the rapid development of cutting-edge technology, the field is transitioning from barcoding individuals to metabarcoding communities. With the advent of next-generation sequencing and a rapid decline in sequencing cost, it has become feasible to assess soil biodiversity at species level. This review article summarizes current approaches in soil biodiversity research along with their advantages and disadvantages.

中文翻译：

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从显微镜到土壤生物多样性评估中的基因组方法。

土壤生物群是地球生物多样性的主要组成部分，200 多年来，显微镜方法是探索它的唯一方法。在过去十年中，基于 DNA 的技术已被应用于土壤生态学。由于尖端技术的快速发展，该领域正在从条形码个人向元条形码社区过渡。随着新一代测序技术的出现和测序成本的快速下降，在物种层面评估土壤生物多样性已变得可行。这篇综述文章总结了当前土壤生物多样性研究的方法及其优缺点。