DNA-binding proteins, including transcription factors, epigenetic and chromatin modifiers, control gene expressions in plants. To pinpoint the binding sits of DNA-binding proteins in genome is crucial for decoding gene regulatory networks. Chromatin immunoprecipitation (ChIP) followed by high-throughput DNA sequencing (ChIP-Seq) is a widely used approach to identify the DNA regions bound by a specific protein in vivo. The information generated from ChIP-Seq has tremendously advanced our understanding on the mechanism of transcription factors, cofactors and histone modifications in regulating gene expression. In this review, we reviewed the recent research advance of ChIP-Seq in plants, including description of the ChIP-Seq workflow and its various applications in plants, and in addition, provided perspective of the potential advances of ChIP-Seq.

中文翻译：

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ChIP-Seq：研究植物中蛋白质-DNA 相互作用的强大工具。

DNA 结合蛋白，包括转录因子、表观遗传和染色质修饰剂，控制植物中的基因表达。确定基因组中 DNA 结合蛋白的结合位点对于解码基因调控网络至关重要。染色质免疫沉淀 (ChIP) 和高通量 DNA 测序 (ChIP-Seq) 是一种广泛使用的方法，用于鉴定体内特定蛋白质结合的 DNA 区域。ChIP-Seq 产生的信息极大地促进了我们对转录因子、辅因子和组蛋白修饰在调节基因表达中的机制的理解。在这篇综述中，我们回顾了 ChIP-Seq 在植物中的最新研究进展，包括对 ChIP-Seq 工作流程及其在植物中的各种应用的描述，此外，还提供了对 ChIP-Seq 潜在进展的展望。