RNA-sequencing technology has been widely adopted to investigate host responses during infection with pathogens. Dual RNA-sequencing (RNA-seq) allows the simultaneous capture of pathogen specific transcripts during infection, providing a more complete view of the interaction. In this review, we focus on the design of dual RNA-seq experiments and the application of downstream data analysis to gain biological insight into both sides of the interaction. Recent literature in this area demonstrates the power of the dual RNA-seq approach and shows that it is not limited to model systems where genomic resources are available. A reduction in sequencing cost and single cell transcriptomics coupled with protein and metabolite level dual approaches are set to enhance our understanding of plant-pathogen interactions. Sequencing costs continue to decrease and single cell transcriptomics is becoming more feasible. In combination with proteomics and metabolomics studies, these technological advances are likely to contribute to our understanding of the temporal and spatial aspects of dynamic plant-pathogen interactions.

中文翻译：

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双 RNA 测序以阐明植物病原体的对决。

RNA测序技术已被广泛用于研究病原体感染期间的宿主反应。双 RNA 测序 (RNA-seq) 允许在感染期间同时捕获病原体特异性转录物，从而提供更完整的相互作用视图。在这篇综述中，我们专注于双 RNA-seq 实验的设计和下游数据分析的应用，以获得对相互作用双方的生物学洞察力。该领域的最新文献证明了双 RNA-seq 方法的强大功能，并表明它不仅限于可用基因组资源的模型系统。测序成本的降低和单细胞转录组学以及蛋白质和代谢物水平的双重方法将增强我们对植物-病原体相互作用的理解。测序成本不断降低，单细胞转录组学变得更加可行。结合蛋白质组学和代谢组学研究，这些技术进步可能有助于我们理解动态植物-病原体相互作用的时间和空间方面。