Next-generation sequencing technologies are revolutionizing biology by permitting, transcriptome sequencing, whole-genome sequencing and resequencing, and genome-wide single nucleotide polymorphism profiling. Orchid research has benefited from this breakthrough, and a few orchid genomes are now available; new biological questions can be approached and new breeding strategies can be designed. The first part of this review describes the unique features of orchid biology. The second part provides an overview of the current next-generation sequencing platforms, many of which are already used in plant laboratories. The third part summarizes the state of orchid transcriptome and genome sequencing and illustrates current achievements. The genetic sequences currently obtained will not only provide a broad scope for the study of orchid biology, but also serves as a starting point for uncovering the mystery of orchid evolution.

中文翻译：

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下一代测序技术在兰花生物学中的高级应用。

下一代测序技术通过许可、转录组测序、全基因组测序和重测序以及全基因组单核苷酸多态性分析正在彻底改变生物学。兰花研究已从这一突破中受益，一些兰花基因组现已可用；可以解决新的生物学问题并设计新的育种策略。本综述的第一部分描述了兰花生物学的独特特征。第二部分概述了当前的下一代测序平台，其中许多已经在植物实验室中使用。第三部分总结了兰花转录组和基因组测序的现状，并说明了目前的成就。目前获得的基因序列不仅将为兰花生物学的研究提供广阔的空间，