Recently the engineered nucleases have revolutionized genome editing to perturb gene expression at specific sites in complex eukaryotic genomes. Three important classes of these genome editing tools are Moreover, the more recent type II Clustered Regularly Inter-spaced Short Palindromic Repeats/Crispr associated protein (CRISPR/Cas9) system has become the most favorite plant genome editing tool for its precision and RNA based specificity unlike its counterparts which rely on protein based specificity. Plasmid-mediated co-delivery of multiple sgRNAs and Cas9 to the Plant cell can simultaneously alter more than one target loci which enable multiplex genome editing. In this review, we discuss recent advancements in the CRISPR/ Cas9 technology mechanism, theory and its applications in plants and agriculture. We also suggest that the CRISPR/ Cas9 as an effective genome editing tool, has vast potential for crop improvement and studying gene regulation mechanism and chromatin remodeling.

中文翻译：

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CRISPR/Cas9 介导的植物基因组编辑时代。

最近，工程核酸酶彻底改变了基因组编辑，以扰乱复杂真核基因组中特定位点的基因表达。这些基因组编辑工具的三个重要类别是此外，最近的 II 型聚集规则间隔短回文重复/Crispr 相关蛋白 (CRISPR/Cas9) 系统因其精确度和基于 RNA 的特异性而成为最受欢迎的植物基因组编辑工具与依赖蛋白质特异性的同类产品不同。质粒介导的多种 sgRNA 和 Cas9 向植物细胞的共同递送可以同时改变多个目标位点，从而实现多重基因组编辑。在这篇综述中，我们讨论了 CRISPR/Cas9 技术机制、理论及其在植物和农业中的应用的最新进展。