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High prevalence of OXA-type carbapenemases among Acinetobacter baumannii strains in a teaching hospital of Tehran.
Acta Microbiologica et Immunologica Hungarica ( IF 1.3 ) Pub Date : 2017-09-05 , DOI: 10.1556/030.64.2017.031
Mandana Zafari 1 , Mohamad Mehdi Feizabadi 2, 3 , Sirous Jafari 4 , Alireza Abdollahi 5 , Azar Sabokbar 1
Affiliation  

Nosocomial infection caused by carbapenem-resistant Acinetobacter baumannii (CRAB) has created a public health concern all around the world. In this study, 100 isolates of CRAB from hospitalized patients during 2015-2016 at Imam Khomeini Hospital were investigated to determine the rates of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains using Kirby-Bauer disk diffusion method. The minimum inhibitory concentrations (MICs) of six antibiotics were determined by broth microdilution method. Multiplex polymerase chain reaction (PCR) was performed to detect blaOXA-51 like and blaOXA-58 like, blaOXA-23 like, and blaOXA-24 like that are encoding resistance to carbapenems. All CRAB isolates were MDR and XDR and 2% of them were pandrug-resistant (PDR), whereas colistin, polymyxin B, and tigecycline were the most effective agents. All isolates were positive for blaOXA-51 like by PCR. The frequency of blaOXA-23 like and blaOXA-24 like was 81% and 22%, respectively. Findings of this study showed that very few therapeutic options remained for the treatment of CRAB infections and blaOXA-23 like is a dominant resistance gene in CRAB at this hospital.

中文翻译:

在德黑兰的一家教学医院中,鲍曼不动杆菌菌株中OXA型碳青霉烯酶的患病率很高。

耐碳青霉烯的鲍曼不动杆菌(CRAB)引起的医院感染已引起全世界的公共卫生关注。在这项研究中,对2015-2016年伊玛目霍梅尼医院(Imam Khomeini Hospital)住院患者的100株CRAB菌株进行了调查,以使用Kirby-Bauer纸片扩散法确定多重耐药(MDR)和广泛耐药(XDR)菌株的比率。肉汤微量稀释法测定了六种抗生素的最低抑菌浓度(MIC)。进行多重聚合酶链反应(PCR)以检测bla OXA-51样和bla OXA-58样,bla OXA-23样和bla OXA-24样编码对碳青霉烯类药物的抗性。所有的CRAB分离物均为MDR和XDR,其中2%为耐泛药(PDR),而大肠菌素,多粘菌素B和替加环素是最有效的药物。通过PCR,所有分离物均为bla OXA-51阳性。bla OXA-23 like和bla OXA-24 like的频率分别为81%和22%。这项研究的发现表明,治疗CRAB感染的方法还很少,bla OXA-23就像这家医院CRAB的主要耐药基因一样
更新日期:2020-08-21
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