Nosocomial infection caused by carbapenem-resistant Acinetobacter baumannii (CRAB) has created a public health concern all around the world. In this study, 100 isolates of CRAB from hospitalized patients during 2015-2016 at Imam Khomeini Hospital were investigated to determine the rates of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains using Kirby-Bauer disk diffusion method. The minimum inhibitory concentrations (MICs) of six antibiotics were determined by broth microdilution method. Multiplex polymerase chain reaction (PCR) was performed to detect bla_{OXA-51 like} and bla_{OXA-58 like}, bla_{OXA-23 like}, and bla_{OXA-24 like} that are encoding resistance to carbapenems. All CRAB isolates were MDR and XDR and 2% of them were pandrug-resistant (PDR), whereas colistin, polymyxin B, and tigecycline were the most effective agents. All isolates were positive for bla_{OXA-51 like} by PCR. The frequency of bla_{OXA-23 like} and bla_{OXA-24 like} was 81% and 22%, respectively. Findings of this study showed that very few therapeutic options remained for the treatment of CRAB infections and bla_{OXA-23 like} is a dominant resistance gene in CRAB at this hospital.

中文翻译：

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在德黑兰的一家教学医院中，鲍曼不动杆菌菌株中OXA型碳青霉烯酶的患病率很高。

耐碳青霉烯的鲍曼不动杆菌（CRAB）引起的医院感染已引起全世界的公共卫生关注。在这项研究中，对2015-2016年伊玛目霍梅尼医院（Imam Khomeini Hospital）住院患者的100株CRAB菌株进行了调查，以使用Kirby-Bauer纸片扩散法确定多重耐药（MDR）和广泛耐药（XDR）菌株的比率。肉汤微量稀释法测定了六种抗生素的最低抑菌浓度（MIC）。进行多重聚合酶链反应（PCR）以检测bla _OXA-51样和bla _OXA-58样，bla _OXA-23样和bla _OXA-24样编码对碳青霉烯类药物的抗性。所有的CRAB分离物均为MDR和XDR，其中2％为耐泛药（PDR），而大肠菌素，多粘菌素B和替加环素是最有效的药物。通过PCR，所有分离物均为bla _OXA-51阳性。bla _{OXA-23 like}和bla _{OXA-24 like}的频率分别为81％和22％。这项研究的发现表明，治疗CRAB感染的方法还很少，bla _OXA-23就像这家医院CRAB的主要耐药基因_一样。