Adhesion of gut bacteria to the intestinal epithelium is the first step in their colonization of the neonatal immature gut. Bacterial colonization of the infant gut is influenced by several factors, of which the most important are the mode of delivery and breast-feeding. Breast-fed infants ingest several grams of human milk oligosaccharides (HMOs) per day, which can become receptor decoys for intestinal bacteria. The most abundant intestinal bacteria in vaginally delivered infants are bifidobacteria, whereas infants born by cesarean section are colonized by clostridia. The influence of HMOs on the adhesion of five strains of intestinal bacteria (three bifidobacterial strains and two clostridial strains) to mucus-secreting and non-mucus-secreting human epithelial cells was investigated. Bifidobacterium bifidum 1 and Bifidobacterium longum displayed almost the same level of adhesion in the presence and absence of HMOs. By contrast, adhesion of Clostridium butyricum 1 and 2 decreased from 14.41% to 6.72% and from 41.54% to 30.91%, respectively, in the presence of HMOs. The results of this study indicate that HMOs affect bacterial adhesion and are an important factor influencing bacterial colonization of the gut. Adhesion of the tested bacteria correlates with their ability to autoaggregate.

中文翻译：

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人乳寡糖对双歧杆菌和梭状芽胞杆菌粘附至细胞系的影响。

肠道细菌对肠上皮的粘附是它们在新生儿未成熟肠道中定殖的第一步。婴儿肠道细菌定植受多种因素影响，其中最重要的是分娩方式和母乳喂养方式。母乳喂养的婴儿每天摄入几克人乳低聚糖（HMO），它们可能成为肠细菌的受体诱饵。阴道分娩婴儿中最丰富的肠道细菌是双歧杆菌，而剖宫产出生的婴儿被梭状芽孢杆菌定殖。研究了HMOs对5种肠道细菌菌株（3种双歧杆菌菌株和2种梭菌菌株）对分泌粘液和非粘液的人类上皮细胞粘附的影响。在存在和不存在HMO的情况下，双歧双歧杆菌1和长双歧杆菌表现出几乎相同的粘附水平。相反，在存在HMO的情况下，丁酸梭菌1和2的粘附力分别从14.41％降低到6.72％和从41.54％降低到30.91％。这项研究的结果表明，HMOs影响细菌粘附，并且是影响肠道细菌定植的重要因素。被测细菌的粘附性与其自动聚集的能力有关。这项研究的结果表明，HMOs影响细菌粘附，并且是影响肠道细菌定植的重要因素。被测细菌的粘附性与其自动聚集的能力有关。这项研究的结果表明，HMOs影响细菌粘附，并且是影响肠道细菌定植的重要因素。被测细菌的粘附性与其自动聚集的能力有关。