Generating techniques to enhance the success of blastomere separation is important for bovine economy, because it increases the number of transferable embryos. This study aimed to identify the optimum cryoprotectants for the vitrification of bovine embryos and the separation of blastomeres at different stages. In experiment 1, expanded blastocysts were vitrified in two different vitrification solutions, either (1) ethylene glycol (EG) + propylene glycol (PG) or (2) EG. The survival rate of blastocysts in the EG + PG was higher than that of the EG. In experiment 2, intact two-cell and eight-cell stage embryos were vitrified in the same solutions used in experiment 1. The EG + PG produced more dead embryos than the EG (P < 0.05). In the EG, the rate of blastocyst formation was similar for the vitrified two- and eight-cell embryos and the non-vitrified ywo-cell embryos. In experiment 3, separated blastomeres of two- and eight-cell embryos were vitrified in EG. There was no difference in the rate of blastocyst formation and total number of cells between the two vitrified groups. In summary, at the blastocyst stage, EG + PG was superior, based on both survival rates and cell numbers; however, at the 2-8 cell stage, the use of EG alone was better than the other groups.

中文翻译：

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牛胚胎在不同发育阶段和在不同溶液中玻璃化后分离的卵裂球的存活和发育能力。

增强卵裂球分离成功率的产生技术对牛经济很重要，因为它增加了可转移胚胎的数量。这项研究旨在为牛胚胎玻璃化和分离卵裂球的不同阶段确定最佳的冷冻保护剂。在实验1中，将膨胀的胚泡在两种不同的玻璃化溶液中进行玻璃化：（1）乙二醇（EG）+丙二醇（PG）或（2）EG。EG + PG中胚泡的存活率高于EG。在实验2中，在与实验1相同的溶液中对完整的两细胞和八细胞阶段胚胎进行了玻璃化。与EG相比，EG + PG产生了更多的死亡胚胎（P <0.05）。在EG中，玻璃化的2细胞和8细胞胚胎和非玻璃化的ywo细胞胚胎的胚泡形成速率相似。在实验3中，将两细胞和八细胞胚胎的分离的卵裂球在EG中玻璃化。两组玻璃化后的胚泡形成速率和细胞总数没有差异。综上所述，在胚泡期，基于存活率和细胞数量，EG + PG更为优越。然而，在2-8个细胞阶段，单独使用EG优于其他组。基于存活率和细胞数量；然而，在2-8个细胞阶段，单独使用EG优于其他组。基于存活率和细胞数量；然而，在2-8个细胞阶段，单独使用EG优于其他组。