The varied nature of human cancers is recapitulated, at least to some extent, in the diverse NCI-60 panel of human cancer cell lines. Here, I used a basic, continuous variable of proliferating cells, their doubling time, to stratify the proteome across the NCI-60 cell lines. Among >7000 proteins quantified in the NCI-60 panel previously, the levels of 84 proteins increase in cells that proliferate slowly. This set overlapped with the hallmark molecular signature "epithelial-mesenchymal transition (EMT)" (p value = 1.1E-07). Conversely, the levels of 105 proteins increased in cells that proliferate faster and overlapped with the molecular signatures for "MYC targets V1" (p value = 3.8E-38) and "E2F targets" (p value = 2.4E-34). These data for the first time identify proteins whose levels are dynamically associated with doubling time, but not necessarily with cancer type origins, and argue for the incorporation of doubling time measurements in cell line-based profiling studies.

中文翻译：

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与NCI-60癌细胞系倍增时间相关的蛋白质。

至少在某种程度上，人类癌症细胞系的各种NCI-60研究小组概括了人类癌症的多样性。在这里，我使用了增殖细胞的基本连续变量（它们的倍增时间）来对NCI-60细胞系中的蛋白质组进行分层。在先前在NCI-60面板中定量的> 7000种蛋白质中，在缓慢增殖的细胞中84种蛋白质的水平增加。该组与标志性分子标记“上皮-间质转化（EMT）”重叠（p值= 1.1E-07）。相反，细胞中105种蛋白质的水平增加，细胞增殖更快，并且与“ MYC靶标V1”（p值= 3.8E-38）和“ E2F靶标”（p值= 2.4E-34）的分子标记重叠。