The cuprizone model is a well-established and investigated paradigm to study demyelination and remyelination in rodents. Cuprizone is usually administrated by mixing in the powdered or pelleted rodent chow. However, since cuprizone is sensitive to the environment and the consumption of it varies between different animals, the major issue is the discrepancy in demyelination of the animals. This study reports the development of the cuprizone model by gavage administrations in mice. Following testing a series of doses of cuprizone, 400 mg/kg/day was found to be the best dosage to induce dramatic and consistent demyelination after 5 weeks of administration; while remyelination quickly occurred after 9 days of cuprizone withdrawal. The advantage of this alternative model is that the consumption of cuprizone could be well controlled, and the mice were exposed to the same dose of cuprizone. Thus, the variation in demyelination was minimized. This alternative cuprizone dosing regime minimizes the interanimal variability on demyelination and hence provides a consistent model for pharmacological evaluations, in addition to reducing the number of animals used in the experiments.

中文翻译：

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铜杯诱导的脱髓鞘和再髓鞘小鼠模型的替代。

铜酮模型是一种成熟的模型，用于研究啮齿动物的脱髓鞘和再髓鞘。通常通过在粉状或颗粒状的啮齿动物食物中混合来施用铜酮。但是，由于铜酮对环境敏感，并且不同动物之间的消耗量也不同，所以主要问题是动物脱髓鞘的差异。这项研究报告了通过管饲法在小鼠体内开发了cuprizone模型的过程。在测试了一系列剂量的cuprizone之后，发现400 mg / kg / day是在给药5周后诱导剧烈而持续的脱髓鞘的最佳剂量。停药9天后很快发生了髓鞘再生。这种替代模型的优点是可以很好地控制铜酮的消耗，并且使小鼠暴露于相同剂量的铜酮。因此，脱髓鞘的变化最小。这种替代的铜酮给药方案最大程度地减少了脱髓鞘作用的动物间差异，因此除了减少了实验中使用的动物数量外，还为药理学评估提供了一致的模型。