Chrysanthemums (Chrysanthemum morifolium) are susceptible to tobacco mosaic virus (TMV). TMV-based expression vectors have been used in high-throughput experiments for production of foreign protein in plants and also expressing green fluorescent protein (GFP) to allow visualization of TMV movement. Here, we used TMV expressing the GFP to examine the infection of chrysanthemum by a TMV-based expression vector. Viral replication, movement and GFP expression by TMV-GFP were verified in upper leaves of chrysanthemums up to 73 days post inoculation (dpi) by RT-PCR. Neither wild-type TMV nor TMV-GFP induced symptoms. GFP fluorescence was seen in the larger veins of the inoculated leaf, in the stem above the inoculation site and in petioles of upper leaves, although there was no consistent detection of GFP fluorescence in the lamina of upper leaves under UV. Thus, a TMV-based expression vector can infect chrysanthemum and can be used for the in vivo study of gene functions.

中文翻译：

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基于烟草花叶病毒的表达载体系统在菊花中的使用。

菊花（Chrysanthemum morifolium）对烟草花叶病毒很敏感（TMV）。基于TMV的表达载体已用于高通量实验中，用于在植物中产生外源蛋白，并表达绿色荧光蛋白（GFP）以可视化TMV运动。在这里，我们使用表达GFP的TMV检查基于TMV的表达载体对菊花的感染。通过RT-PCR在接种后长达73天（dpi）验证了菊花上部叶中病毒复制，运动和由TMV-GFP表达的GFP。野生型TMV和TMV-GFP均未引起症状。GFP荧光可见于接种叶的较大静脉中，接种部位上方的茎中和上部叶片的叶柄中，尽管在紫外线下未一致检测到上部叶片的GFP荧光。因此，基因功能的体内研究。