Tomato yellow leaf curl virus (TYLCV) infection of the common bean (Phaseolus vulgaris) has been reported, but soybean (Glycine max) has not previously been identified as a TYLCV host. Five cultivars of white soybean were agro-inoculated using an infectious TYLCV clone. At 30 days post-inoculation, they showed infection rates of 25% to 100%. Typical TYLCV symptoms were not observed in any inoculated plants. To examine whether TYLCV was transmitted in soybean seeds, DNA was isolated from bundles of five randomly selected seeds from TYLCV-inoculated soybean plants and amplified with a TYLCV-specific primer set. With the exception of one bundle, all bundles of seeds were verified to be TYLCV-infected. Virus dissemination was also confirmed in three of the 14 bunches. Viral replication was also identified in seeds and seedlings. This is the first report demonstrating that soybean is a TYLCV host, and that TYLCV is a seed-transmissible virus in white soybean.

中文翻译：

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白大豆（Glycine max）中番茄黄曲叶病毒的种子传播。


番茄黄曲叶病毒(TYLCV) 感染普通豆类 ( Phaseolus vulgaris ) 已有报道，但大豆 ( Glycine max ) 此前尚未被确定为 TYLCV 宿主。使用感染性 TYLCV 克隆对五个白大豆品种进行农业接种。接种后 30 天，感染率达到 25% 至 100%。在任何接种的植物中均未观察到典型的 TYLCV 症状。为了检查 TYLCV 是否在大豆种子中传播，从接种 TYLCV 的大豆植物中随机选择的五粒种子中分离出 DNA，并用 TYLCV 特异性引物组进行扩增。除一捆种子外，所有种子捆均被证实感染 TYLCV。 14 组中的 3 组也证实了病毒传播。种子和幼苗中也发现了病毒复制。这是第一份证明大豆是 TYLCV 宿主的报告，并且 TYLCV 是白大豆中的种子传播病毒。