Efforts to control viral diseases in crop production include several types of physical or chemical treatments; antiviral extracts of a number of plants have also been examined to inhibit plant viral infection. However, treatments utilizing naturally selected microorganisms with activity against plant viruses are poorly documented. Here we report isolation of a soil inhabiting bacterium, Pseudomonas oleovorans strain KBPF-004 (developmental code KNF2016) which showed antiviral activity against mechanical transmission of tobamoviruses. Antiviral activity was also evaluated in seed transmission of two tobamoviruses, Pepper mild mottle virus (PMMoV) and Cucumber green mottle mosaic virus (CGMMV), by treatment of seed collected from infected pepper and watermelon, respectively. Pepper and watermelon seeds were treated with culture supernatant of P. oleovorans strain KBPF-004 or control strain ATCC 8062 before planting. Seeds germinated after treatment with water or ATCC 8062 yielded about 60% CGMMV or PMMoV positive plants, whereas < 20% of KBPF-004-treated seeds were virus-infected, a significantly reduced seed transmission rate. Furthermore, supernatant of P. oleovorans strain KBPF-004 remodeled aggregation of PMMoV 126 kDa protein and subcellular localization of movement protein in Nicotiana benthamiana, diminishing aggregation of the 126 kDa protein and essentially abolishing association of the movement protein with the microtubule network. In leaves agroinfiltrated with constructs expressing the coat protein (CP) of either PMMoV or CGMMV, less full-size CP was detected in the presence of supernatant of P. oleovorans strain KBPF-004. These changes may contribute to the antiviral effects of P. oleovorans strain KBPF-004.

中文翻译：

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假单胞菌菌株KBPF-004培养上清降低了黄瓜绿斑驳花叶病毒和胡椒轻斑驳病毒的种子传播，并重塑了126 kDa的聚集体和胡椒轻斑驳病毒的运动蛋白亚细胞定位。

在作物生产中控制病毒性疾病的努力包括几种物理或化学处理方法。还检查了许多植物的抗病毒提取物以抑制植物病毒感染。然而，利用自然选择的具有抗植物病毒活性的微生物进行治疗的文献报道很少。在这里，我们报告了一种土壤居住细菌油假单胞菌菌株KBPF-004（开发代码KNF2016）的分离，该菌株对烟草花叶病毒的机械传播具有抗病毒活性。还通过两种烟草花叶病毒，胡椒轻斑驳病毒（PMMoV）和黄瓜绿斑驳花叶病毒的种子传播评估了抗病毒活性。（CGMMV），分别处理从感染的胡椒和西瓜中收集的种子。辣椒和西瓜种子在播种前用油酸假单胞菌菌株KBPF-004或对照菌株ATCC 8062的培养上清液处理。用水或ATCC 8062处理后发芽的种子产生约60％的CGMMV或PMMoV阳性植物，而KBPF-004处理的种子中少于20％的种子被病毒感染，大大降低了种子的传播率。此外，上清液P.食油假单胞菌菌株的PMMoV 126 kDa蛋白的KBPF-004改装聚集和移动蛋白的亚细胞定位本塞姆氏烟草，减少了126 kDa蛋白的聚集，并基本消除了运动蛋白与微管网络的结合。在用表达PMMoV或CGMMV的外壳蛋白（CP）的构建体农杆菌浸润的叶子中，在油橄榄假单胞菌KBPF-004菌株的上清液的存在下，检测到的全尺寸CP较少。这些变化可能有助于油橄榄假单胞菌KBPF-004的抗病毒作用。