The present study was conducted to evaluate the possibility of using in vitro-produced parthenogenetic (PA) embryos for co-transfer with morulae that had been collected in vivo and cryopreserved. The proportion of PA blastocysts (20.5%) was higher than that of their in vitro fertilization (IVF) counterparts (16.6%). Although there were no differences in morphology or diameter between the two groups, the number of cells in early PA blastocysts after in vitro culture for 6 days was lower than for IVF blastocysts (25.7 and 30.4 cells, respectively), and the number in recovered PA blastocysts was also smaller than that in recovered IVF blastocysts (37.4 and 50.2 cells, respectively). When 10 morulae warmed after vitrification were co-transferred with 10 PA blastocysts (total 20 embryos) to the uterus of five recipients, the rates of pregnancy and farrowing did not differ, but the average period until spontaneous abortion tended to be longer relative to the control (when 20 morulae were transferred). These data suggest that in vitro-produced PA embryos offer the possibility of assisted pregnancy for cryopreserved embryos; further experiments will be needed to confirm the beneficial effect of this approach on piglet production.

中文翻译：

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利用猪体外产生的孤雌生殖胚胎与玻璃化和温暖的胚胎共转移。

进行本研究以评估使用体外产生的孤雌生殖（PA）胚胎与已在体内收集并冷冻保存的桑ula共转移的可能性。PA胚泡的比例（20.5％）高于体外受精（IVF）的比例（16.6％）。尽管两组之间的形态或直径没有差异，但体外培养6天后，早期PA胚泡的细胞数量比IVF胚泡（分别为25.7和30.4个细胞）少，并且回收的PA数量胚泡也小于回收的IVF胚泡（分别为37.4和50.2个细胞）。将玻璃化后变暖的10个桑ula与10个PA胚泡（总共20个胚胎）共同转移到5个受体的子宫中，妊娠率和分娩率没有差异，但是自发流产的平均时间相对于对照组要长（转移了20桑ula）。这些数据表明，体外产生的PA胚胎为冷冻保存的胚胎提供了辅助妊娠的可能性。需要进一步的实验来确认这种方法对仔猪生产的有益作用。