Stem cells from human exfoliated deciduous teeth (SHED) are isolated from the dental pulp tissue of primary teeth and can differentiate into neuronal cells. Although SHED are a desirable type of stem cells for transplantation therapy and for the study of neurological diseases, a large part of the neuronal differentiation machinery of SHED remains unclear. Recent studies have suggested that mitochondrial activity is involved in the differentiation of stem cells. In the present work, we investigated the neuronal differentiation machinery of SHED by focusing on mitochondrial activity. During neuronal differentiation of SHED, we observed increased mitochondrial membrane potential, increased mitochondrial DNA, and elongated mitochondria. Furthermore, to examine the demand for mitochondrial activity in neuronal differentiation, we then differentiated SHED into neuronal cells in the presence of rotenone, an inhibitor of mitochondrial respiratory chain complex I, and carbonyl cyanide m-chlorophenyl hydrazone (CCCP), a mitochondrial uncoupler, and found that neuronal differentiation was inhibited by treatment with rotenone and CCCP. These results indicated that increased mitochondrial activity was crucial for the neuronal differentiation of SHED.Key words: mitochondria, differentiation, stem cells, dental pulp, exfoliated deciduous teeth.

中文翻译：

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线粒体调节人脱落的乳牙中干细胞的分化。

从脱落的乳牙（SHED）的干细胞是从乳牙的牙髓组织中分离出来的，可以分化为神经元细胞。尽管SHED是用于移植疗法和神经疾病研究的理想干细胞类型，但SHED的神经元分化机制的很大一部分仍不清楚。最近的研究表明，线粒体活性与干细胞的分化有关。在目前的工作中，我们通过关注线粒体的活性研究了SHED的神经元分化机制。在SHED的神经元分化过程中，我们观察到线粒体膜电位增加，线粒体DNA增加和线粒体延长。此外，要检查神经元分化过程中线粒体活性的需求，然后，我们在鱼藤酮（线粒体呼吸链复合体I的抑制剂）和线粒体解偶联剂羰基氰化物间氯苯基（CCCP）的存在下，将SHED分化为神经元细胞，发现鱼藤酮和CCCP处理可抑制神经元分化。 。这些结果表明线粒体活性的增加对于SHED的神经元分化至关重要。关键词：线粒体，分化，干细胞，牙髓，脱落的乳牙。