Objectives: This study investigated the potential anti-inflammatory effect of hesperidin against carrageenan induced pleurisy in rat model.

Methods: Twenty-four adult female Wistar rats (350 - 450g) were grouped as follows: Group I: rats were administered saline solution only (Normal control group); Group II: rats were administered saline solution (NaCl 0.9%) orally and injected with carrageenan (Inflammation control group); Group III: rats were administered hesperidin only (Hesperidin group); Group IV: rats were administered hesperidin orally and intrapleurally injected with 2% carrageenan (Inflammation treated with hesperidin group). The exudate volume, total leukocyte count, reactive oxygen species (ROS), myeloperoxidase (MPO),δ–aminolevulinate dehydratase (δ-ALA-D), catalase (CAT), superoxide dismutase (SOD), activities as well as non-protein thiol group (NPSH) and thiobarbituric acid reactive substances (TBARS) levels were determined.

Results: Pretreatment with hesperidin at a dose of 80 mg/kg orally per day for 21 days, minimized the increase in pleural exudate volume and leucocyte count and modulated the activities of MPO, SOD and CAT, as well as the levels of ROS, NPSH and TBARS in carrageenan-induced rats.

Conclusion: Our results suggest that hesperidin can elicit its anti-inflammatory action by blocking exudate and leukocyte influx into pleural cavity, inhibiting MPO activity and preventing oxidative damage.

目的：本研究探讨了橙皮苷对大鼠模​​型中角叉菜胶诱导的胸膜炎的潜在抗炎作用。

方法：将24只成年雌性Wistar大鼠（350～450g）分组如下： I组：仅给予生理盐水（正常对照组）；第二组：大鼠口服生理盐水（NaCl 0.9%），注射角叉菜胶（炎症对照组）；第三组：仅给予大鼠橙皮苷（橙皮苷组）；第四组：给大鼠口服橙皮苷，胸腔内注射2%角叉菜胶（橙皮苷治疗炎症组）。渗出液量、白细胞总数、活性氧（ROS）、髓过氧化物酶（MPO）、δ-氨基乙酰丙酸脱水酶（δ-ALA-D）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、活性以及非蛋白测定了硫醇基（NPSH）和硫代巴比妥酸反应物质（TBARS）的水平。

结果：橙皮苷以 80 mg/kg 的剂量每天口服 21 天，最大限度地减少胸腔渗出液量和白细胞计数的增加，并调节 MPO、SOD 和 CAT 的活性以及 ROS、NPSH 的水平和 TBARS 在角叉菜胶诱导的大鼠中。

结论：我们的研究结果表明，橙皮苷可通过阻断渗出液和白细胞流入胸膜腔、抑制 MPO 活性和防止氧化损伤来引发其抗炎作用。