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Role of pH in the recovery of bovine milk oligosaccharides from colostrum whey permeate by nanofiltration
International Dairy Journal ( IF 3.1 ) Pub Date : 2017-03-01 , DOI: 10.1016/j.idairyj.2016.10.016
Joshua L Cohen 1 , Daniela Barile 1, 2 , Yan Liu 1 , Juliana M L N de Moura Bell 1
Affiliation  

Milk oligosaccharides are associated with improved health outcomes in infants. Nanofiltration (NF) is used for isolation of bovine milk oligosaccharides (BMO). The study aim was to improve the recovery of BMO from lactose-hydrolyzed colostrum whey permeate. The retention factors of carbohydrates at various pH and transmembrane pressures were determined for a nanofiltration membrane, which was used at pilot scale to purify BMO. Carbohydrates were quantified by liquid chromatography and characterized using nano-LC-Chip-QToF mass spectrometry. BMO purity was improved from an initial 4% in colostrum whey permeate to 98%, with 99.8% permeation of monosaccharides and 96% recovery of oligosaccharides, represented by 23 unique BMO compounds identified in the final retentate. The pH during NF was a determining factor in the selectivity of carbohydrate separation. This NF method can be applied to conventional cheese-whey permeate and other milk types for extraction of bioactive oligosaccharides providing new options for the dairy industry.

中文翻译:

pH 在通过纳滤从初乳乳清渗透物中回收牛乳低聚糖中的作用

牛奶寡糖与改善婴儿的健康结果有关。纳滤 (NF) 用于分离牛乳寡糖 (BMO)。该研究的目的是提高从乳糖水解初乳乳清渗透物中回收 BMO。确定了纳米过滤膜在不同 pH 值和跨膜压力下的碳水化合物保留因子,该膜用于中试规模以纯化 BMO。碳水化合物通过液相色谱法定量,并使用纳米 LC-Chip-QToF 质谱法进行表征。BMO 纯度从初乳乳清渗透物中的最初 4% 提高到 98%,单糖渗透率为 99.8%,寡糖回收率为 96%,由最终保留物中鉴定的 23 种独特的 BMO 化合物代表。NF 过程中的 pH 值是碳水化合物分离选择性的决定因素。这种 NF 方法可应用于传统的奶酪乳清渗透液和其他牛奶类型,用于提取生物活性低聚糖,为乳制品行业提供新的选择。
更新日期:2017-03-01
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