Methanobrevibacter arboriphilus strain DH1 is an autotrophic methanogen that was isolated from the wetwood of methane-emitting trees. This species has been of considerable interest for its unusual oxygen tolerance and has been studied as a model organism for more than four decades. Strain DH1 is closely related to other host-associated Methanobrevibacter species from intestinal tracts of animals and the rumen, making this strain an interesting candidate for comparative analysis to identify factors important for colonizing intestinal environments. Here, the genome sequence of M. arboriphilus strain DH1 is reported. The draft genome is composed of 2.445.031 bp with an average GC content of 25.44% and predicted to harbour 1964 protein-encoding genes. Among the predicted genes, there are also more than 50 putative genes for the so-called adhesin-like proteins (ALPs). The presence of ALP-encoding genes in the genome of this non-host-associated methanogen strongly suggests that target surfaces for ALPs other than host tissues also need to be considered as potential interaction partners. The high abundance of ALPs may also indicate that these types of proteins are more characteristic for specific phylogenetic groups of methanogens rather than being indicative for a particular environment the methanogens thrives in.

中文翻译：

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非宿主相关的嗜甲烷短杆菌菌株 DH1 的基因组草图编码大量粘附素样蛋白。


Methanobrevibacter arboriphilus菌株 DH1 是一种自养产甲烷菌，是从排放甲烷的树木的湿材中分离出来的。该物种因其不同寻常的耐氧性而引起人们极大的兴趣，并作为模式生物进行了四十多年的研究。菌株 DH1 与来自动物肠道和瘤胃的其他宿主相关甲烷短杆菌属密切相关，使该菌株成为比较分析的有趣候选者，以确定对定植肠道环境重要的因素。在此，报告了M. arboriphilus菌株 DH1 的基因组序列。基因组草图由 2.445.031 bp 组成，平均 GC 含量为 25.44%，预计包含 1964 个蛋白质编码基因。在预测的基因中，还有 50 多个所谓的粘附素样蛋白 (ALP) 的推定基因。这种非宿主相关产甲烷菌基因组中 ALP 编码基因的存在强烈表明，除了宿主组织之外，ALP 的目标表面也需要被视为潜在的相互作用伙伴。高丰度的 ALP 也可能表明，这些类型的蛋白质更具有产甲烷菌特定系统发育群体的特征，而不是指示产甲烷菌生长的特定环境。