The oleaginous yeast Yarrowia lipolytica is a convenient model for investigating lipid biosynthesis and for engineering high lipid accumulated strains. In this organism, the pentose phosphate pathway is the major source of NADPH for lipid biosynthesis. Thus, we over-expressed gene encoding NADP+-dependent glucose-6-phosphate dehydrogenase (ZWF1) in a strain deficient in peroxisome biogenesis. However, this strategy suppressed growth during cultivation under lipogenic conditions and did not significantly increase lipid accumulation. Remarkably, co-expression of gene encoding acyl-CoA binding protein (ACBP), which functions as an intracellular acyl-CoA transporter and acyl-CoA-pool former, restored growth. Co-expression of ZWF1 and ACBP increased the lipid content to 30% of dry cell weight via de novo lipid synthesis. In comparison to wild type, the engineered strain accumulated 41% more lipids with a higher ratio of saturated to unsaturated fatty acids.

中文翻译：

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6-磷酸葡萄糖脱氢酶和酰基辅酶A结合蛋白的共表达增强了酵母解脂耶氏酵母中的脂质积累

产油酵母解脂耶氏酵母是研究脂质生物合成和工程高脂质积累菌株的便捷模型。在该生物体中，磷酸戊糖途径是用于脂质生物合成的 NADPH 的主要来源。因此，我们在过氧化物酶体生物发生缺陷的菌株中过度表达了编码 NADP+ 依赖性葡萄糖-6-磷酸脱氢酶 (ZWF1) 的基因。然而，这种策略抑制了脂肪生成条件下培养过程中的生长，并且没有显着增加脂质积累。值得注意的是，编码酰基辅酶A结合蛋白（ACBP）的基因的共表达恢复了生长，该蛋白起细胞内酰基辅酶A转运蛋白和酰基辅酶A池形成剂的作用。ZWF1 和 ACBP 的共表达通过从头脂质合成将脂质含量增加到细胞干重的 30%。与野生型相比，