OBJECTIVE The extracellular matrix protein reelin regulates early brain development and synaptic plasticity in adulthood. Reelin is decreased in the postmortem brain in schizophrenia patients. Reelin's two receptors, ApoER2 and VLDLR, are also substrates for ApoE - a key lipoprotein that regulates phospholipid homeostasis in the brain. The goal of the present study was therefore to examine phospholipids and their constituent fatty acids, and determine whether there is an association between reelin, its receptors and phospholipids in the brain. METHODS Dorsolateral prefrontal cortex (BA9) grey matter was obtained from the Stanley Foundation Neuropathology Consortium. Samples included tissue from 35 controls, 35 schizophrenia and 34 bipolar disorder patients. Phospholipids were measured using gas liquid chromatography. RESULTS We quantified 15 individual fatty acid or plasmalogen species for phosphatidylethanolamine and phosphatidylcholine fractions, each comprising >0.5% of the total fatty acid pool. There were no group differences in phospholipids or individual fatty acid species after correcting for multiple comparisons. However, for the entire cohort, both the polyunsaturated subclass of fatty acids, and ApoE, correlated significantly with reelin expression, with a number of individual ω-6 fatty acid species also demonstrating a significant positive correlation. There was a non-significant trend for similar effects with VLDLR expression as for reelin. CONCLUSION Phospholipids and fatty acids in the dorsolateral cortex do not differ in patients with schizophrenia, bipolar disorder and controls. Reelin expression in this brain region is associated with polyunsaturated fatty acids and ApoE, suggesting further study of potential physiological interactions between these substrates is warranted.

中文翻译：

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精神分裂症和双相情感障碍的前额叶脂肪酸组成：与 reelin 表达的关联

目的 细胞外基质蛋白 reelin 调节成年早期大脑发育和突触可塑性。Reelin 在精神分裂症患者的死后大脑中减少。Reelin 的两个受体 ApoER2 和 VLDLR 也是 ApoE 的底物，ApoE 是一种调节大脑中磷脂稳态的关键脂蛋白。因此，本研究的目标是检查磷脂及其组成脂肪酸，并确定 reelin、其受体和大脑中的磷脂之间是否存在关联。方法 背外侧前额叶皮层 (BA9) 灰质来自 Stanley Foundation Neuropathology Consortium。样本包括来自 35 名对照者、35 名精神分裂症患者和 34 名双相情感障碍患者的组织。使用气液色谱法测量磷脂。结果 我们量化了 15 种单独的脂肪酸或缩醛磷脂种类的磷脂酰乙醇胺和磷脂酰胆碱成分，每种成分均占总脂肪酸池的 0.5% 以上。校正多重比较后，磷脂或单个脂肪酸种类没有组间差异。然而，对于整个队列，多不饱和脂肪酸亚类和 ApoE 都与 reelin 表达显着相关，许多单独的 ω-6 脂肪酸种类也显示出显着的正相关。VLDLR 表达与 reelin 的类似效果没有显着趋势。结论精神分裂症、双相情感障碍患者和对照组的背外侧皮层中的磷脂和脂肪酸没有差异。