Background

Reactive Red 31, applied extensively in the commercial textile industry, is a hazardous and persistent azo dye compound often present in dye manufacturing and textile industrial effluents. Aspergillus bombycis strain was isolated from dye contaminated zones of Gujarat Industrial Development Corporation, Vatva, Ahmedabad, India. The decolorization potential was monitored by the decrease in maximum absorption of the dye using UV–visible spectroscopy. Optimization of physicochemical conditions was carried out to achieve maximum decolorization of Reactive Red 31 by fungal pellets.

Results

Pellets of A. bombycis strain were found to decolorize this dye (20 mg/L) under aerobic conditions within 12 h. The activity of azoreductase, laccase, phenol oxidase and Manganese peroxidase in fungal culture after decolorization was about 8, 7.5, 19 and 23.7 fold more than before decolorization suggesting that these enzymes might be induced by the addition of Reactive Red 31 dye, and thus results in a higher decolorization. The lab-scale reactor was developed and mineralization of Reactive Red 31 dye by fungal pellets was studied at 6, 12 and 24 h of HRT (hydraulic retention time). At 12 h of HRT, decolorization potential, chemical oxygen demand (COD) and total organic carbon reduction (TOC) was 99.02, 94.19, and 83.97%, respectively, for 20 mg/L of dye concentration.

Conclusions

Dye decolorization potential of A. bombycis culture was influenced by several factors such as initial dye concentration, biomass concentration, pH, temperature, and required aerated conditions. Induction of azoreductase, laccase, phenol oxidase, and Mn-peroxidase enzymes was observed during dye decolorization phase. A. bombycis pellets showed potential in mineralization of dye in the aerobic reactor system. Isolated fungal strain A. bombycis showed better dye decolorization performance in short duration of time (12 h) as compared to other reported fungal cultures.

Degradation of RR31 dye in developed aerobic fungal pelleted reactor.

中文翻译：

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使用家蚕曲霉颗粒对模拟废水中的磺化纺织染料活性红 31 进行矿化。

 背景

活性红 31 广泛应用于商业纺织工业，是一种危险且持久的偶氮染料化合物，通常存在于染料制造和纺织工业废水中。家蚕曲霉菌菌株是从印度艾哈迈达巴德瓦特瓦古吉拉特邦工业发展公司的染料污染区分离出来的。使用紫外-可见光谱通过染料最大吸收的减少来监测脱色潜力。对物理化学条件进行了优化，以实现真菌颗粒对活性红 31 的最大脱色。

 结果

发现家蚕菌株的颗粒在有氧条件下 12 小时内使该染料 (20 mg/L) 脱色。脱色后真菌培养物中偶氮还原酶、漆酶、酚氧化酶和锰过氧化物酶的活性比脱色前增加约8、7.5、19和23.7倍，表明这些酶可能是通过添加活性红31染料诱导的，因此结果具有较高的脱色度。开发了实验室规模的反应器，并在 6、12 和 24 小时的 HRT（水力保留时间）下研究了真菌颗粒对活性红 31 染料的矿化。 HRT 12 h 时，染料浓度为 20 mg/L 时，脱色潜力、化学需氧量 (COD) 和总有机碳还原率 (TOC) 分别为 99.02、94.19 和 83.97%。

 结论

家蚕培养物的染料脱色潜力受到多种因素的影响，例如初始染料浓度、生物量浓度、pH、温度和所需的通气条件。在染料脱色阶段观察到偶氮还原酶、漆酶、酚氧化酶和锰过氧化物酶的诱导。家蚕颗粒在好氧反应器系统中显示出染料矿化的潜力。与其他报道的真菌培养物相比，分离的真菌菌株家蚕在短时间内（12 小时）表现出更好的染料脱色性能。

RR31 染料在开发的好氧真菌颗粒反应器中的降解。