Yeast adhesion to and biofilm formation on surfaces is present in many different environments. In food industry, biofilms may be a source of contaminations, causing food spoilage and reducing quality of products. Candida and Pichia are two common yeast genera involved in the spoilage of some food products. The aim of this study is to assess the potential of Candida and Pichia species to adhere to two types of wooden surfaces (smooth and rough), one of the materials typical for the food processing industry, and investigate the influence of surface roughness of wood on the degree of yeast adhesion. The adhesion of the cells to the wooden surfaces was determined by rinsing them from the surface, followed by methylene blue staining, and quantification after imaging under microscope by automatic counting of viable cells. The results showed that all Candida and Pichia strains were able to adhere to the wooden surfaces in a species- and strain-dependent manner. On the other hand, our data indicated that adhesion by these yeasts was not significantly affected by the roughness of the wood surfaces.

中文翻译：

---

念珠菌属的粘附。和毕赤酵母属。到木质表面。


酵母在表面上的粘附和生物膜的形成存在于许多不同的环境中。在食品工业中，生物膜可能是污染源，导致食品腐败并降低产品质量。念珠菌和毕赤酵母是两种常见的酵母属，与某些食品的腐败有关。本研究的目的是评估念珠菌和毕赤酵母菌粘附在两种类型的木质表面（光滑和粗糙）（食品加工行业典型材料之一）上的潜力，并研究木材表面粗糙度对木材表面粗糙度的影响。酵母的粘附程度。通过将细胞从表面冲洗掉，然后进行亚甲蓝染色，并在显微镜下成像后通过自动计数活细胞进行定量来确定细胞对木质表面的粘附。结果表明，所有念珠菌和毕赤酵母菌株都能以物种和菌株依赖性方式粘附在木质表面上。另一方面，我们的数据表明，这些酵母的粘附并未受到木材表面粗糙度的显着影响。