The benefits of probiotic bacteria have been widely explored. However, fermented foods and digestive system of humans and animals are an inexhaustible source of new potentially probiotic microorganisms. In this study we present three new Lactobacillus plantarum strains isolated from different dairy products: cow's cheese, sheep's cheese and whey. In order to determine the antibacterial activity of yet unexplored L. plantarum strains against Salmonella enterica serotype Typhimurium, in vitro competition and co-culture tests were done. Furthermore, adhesion of these strains to Caco-2 cells and their influence on the adhesion of Salmonella were tested. Results showed the potential probiotic activity of isolated strains. L. plantarum strains survived in the presence of 1% bile salts, they possessed acidification ability, antibacterial activity and significantly attenuated the growth of S. Typhimurium in brain heart infusion broth. All tested L. plantarum strains were able to adhere to Caco-2 cells and significantly impair the adhesion of S. Typhimurium. All three L. plantarum strains exhibited significant probiotic potential and anti-Salmonella activity; therefore, further testing on in vivo models should follow.

中文翻译：

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益生菌工具箱中的三种新的植物乳杆菌菌株，可抵抗肠道病原体肠炎沙门氏菌血清型鼠伤寒。

益生菌的益处已被广泛探索。然而，人类和动物的发酵食品和消化系统是新的潜在益生菌微生物的不竭来源。在这项研究中，我们介绍了从不同乳制品中分离出的三种新的植物乳杆菌菌株：牛奶酪，羊奶酪和乳清。为了确定尚未探索的植物乳杆菌对肠炎沙门氏菌血清型鼠伤寒沙门氏菌的抗菌活性，进行了体外竞争和共培养试验。此外，这些菌株对Caco-2细胞的粘附及其对沙门氏菌粘附的影响经过测试。结果显示了分离菌株的潜在益生菌活性。植物乳杆菌在1％的存在的胆汁盐存活株，他们拥有酸化能力，抗菌活性和显著减弱的生长S.鼠伤寒沙门氏菌在脑心脏浸液肉汤。所有测试的植物乳杆菌菌株能够粘附到Caco-2细胞和显著损害的粘附S.鼠伤寒沙门氏菌。所有三种植物乳杆菌菌株均显示出显着的益生菌潜力和抗沙门氏菌活性。因此，应随后在体内模型上进行进一步测试。