The medicinal plant Hyoscyamus reticulatus L. is a rich source of hyoscyamine and scopolamine, the tropane alkaloids. The use of hairy root cultures has focused significant attention on production of important metabolites such as stable tropane alkaloid production. Elicitation is an effective approach to induce secondary metabolite biosynthetic pathways. Hairy roots were derived from cotyledon explants inoculated with Agrobacterium rhizogenes and elicited by iron oxide nanoparticles (FeNPs) at different concentrations (0, 450, 900, 1800, and 3600 mg L-1) for different exposure times (24, 48, and 72 h). The highest hairy root fresh and dry weights were found in the medium supplemented with 900 mg L-1 FeNPs. Antioxidant enzyme activity was significantly increased in induced hairy roots compared to non-transgenic roots. The highest hyoscyamine and scopolamine production (about fivefold increase over the control) was achieved with 900 and 450 mg L-1 FeNPs at 24 and 48 h of exposure time, respectively. This is the first report of the effect of FeNP elicitor on hairy root cultures of a medicinal plant. We suggest that FeNPs could be an effective elicitor in hairy root cultures in order to increase tropane alkaloid production.

中文翻译：

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氧化铁纳米颗粒诱导基因转化的天仙子根培养物提高天仙子胺和东莨菪碱的产量。

药用植物天仙子胺和东莨菪碱（托烷生物碱）的丰富来源。毛状根培养物的使用已将重点放在重要代谢物的生产上，例如稳定的托烷生物碱的生产。诱导是诱导次级代谢物生物合成途径的有效方法。毛状根来源于接种发根农杆菌的子叶外植体，并通过不同浓度（0、450、900、1800 和 3600 mg L-1）的氧化铁纳米粒子（FeNP）在不同的暴露时间（24、48 和 72）下诱导产生毛状根。 H）。在补充有 900 mg L-1 FeNP 的培养基中发现了最高的毛状根鲜重和干重。与非转基因根相比，诱导毛状根中的抗氧化酶活性显着增加。在暴露时间为 24 小时和 48 小时时，分别使用 900 毫克 L-1 FeNP 和 450 毫克 L-1 FeNP 实现了最高的天仙子胺和东莨菪碱产量（比对照增加约五倍）。这是首次报道 FeNP 诱导子对药用植物毛状根培养物的影响。我们认为，FeNPs 可能是毛状根培养物中的有效诱导剂，以增加托烷生物碱的产量。