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Degeneration pattern in somatic embryos of Pinus sylvestris L.
In Vitro Cellular & Developmental Biology - Plant ( IF 2.2 ) Pub Date : 2017-05-30 , DOI: 10.1007/s11627-016-9797-y
Malin Abrahamsson 1 , Silvia Valladares 1, 2 , Irene Merino 1 , Emma Larsson 1 , Sara von Arnold 1
Affiliation  

Somatic embryos can be used for propagating forest trees vegetatively, which is of great importance for capturing the genetic gain in breeding programs. However, many economically important Pinus species are difficult or impossible to propagate via somatic embryogenesis. In order to get a better understanding of the difficulties to propagate Pinus species via somatic embryogenesis, we are studying the developmental pathway of somatic embryos in different cell lines. In a previous study, we showed that the morphology of early somatic embryos in Scots pine (Pinus sylvestris) differs between cell lines giving rise to normal or abnormal cotyledonary embryos. In this study, we have compared the proliferation and degeneration pattern of early and late embryos in a normal and abnormal cell line. In both cell lines, a high frequency of the embryos degenerated. Among the degenerating embryos, two main degeneration patterns could be distinguished. In the normal cell line, the embryos degenerated similar to how the subordinate embryos are degraded in the seed. In the abnormal cell line, the degeneration of the embryos resulted in a continuous loop of embryo degeneration and differentiation of new embryos. We observed a similar degeneration pattern when embryogenic tissue was initiated from megagametophytes containing zygotic embryos at the stage of cleavage polyembryony. Based on our results, we suggest that the degeneration pattern in abnormal cell lines starts during initiation of embryogenic cultures.

中文翻译:

樟子松体细胞胚的退化模式。

体细胞胚可用于无性繁殖林木,这对于在育种程序中捕获遗传增益非常重要。然而,许多经济上重要的松属物种很难或不可能通过体细胞胚发生繁殖。为了更好地了解通过体细胞胚发生繁殖松属物种的困难,我们正在研究体细胞胚在不同细胞系中的发育途径。在先前的研究中,我们发现苏格兰松(Pinus sylvestris)的早期体细胞胚的形态在细胞系之间有所不同,从而导致正常或异常的子叶胚。在这项研究中,我们比较了正常和异常细胞系中早期和晚期胚胎的增殖和变性模式。在两种细胞系中 高频率的胚胎退化。在退化的胚胎中,可以区分出两种主要的退化模式。在正常细胞系中,胚胎的退化类似于种子中从属胚胎的降解方式。在异常细胞系中,胚胎的变性导致胚胎变性和新胚胎分化的连续循环。当从卵裂多胚阶段包含合子胚的大型配子体启动胚发生组织时,我们观察到了类似的变性模式。根据我们的结果,我们建议异常细胞系的变性模式始于胚胎培养的开始。胚胎的退化类似于种子中从属胚胎的降解方式。在异常细胞系中,胚胎的变性导致胚胎变性和新胚胎分化的连续循环。当从卵裂多胚阶段包含合子胚的大型配子体启动胚发生组织时,我们观察到了相似的变性模式。根据我们的结果,我们建议异常细胞系的变性模式始于胚胎培养的开始。胚胎的退化类似于种子中从属胚胎的降解方式。在异常细胞系中,胚胎的变性导致胚胎变性和新胚胎分化的连续循环。当从卵裂多胚阶段包含合子胚的大型配子体启动胚发生组织时,我们观察到了类似的变性模式。根据我们的结果,我们建议异常细胞系的变性模式始于胚胎培养的开始。当从卵裂多胚阶段包含合子胚的大型配子体启动胚发生组织时,我们观察到了类似的变性模式。根据我们的结果,我们建议异常细胞系的变性模式始于胚胎培养的开始。当从卵裂多胚阶段包含合子胚的大型配子体启动胚发生组织时,我们观察到了相似的变性模式。根据我们的结果,我们建议异常细胞系的变性模式始于胚胎培养的开始。
更新日期:2019-11-01
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