Mutations are heritable changes in the nucleotide sequence of DNA that can lead to many adverse effects. Genotoxicity assays have been used to identify chemical mutagenicity. Recently, next generation sequencing (NGS) has been used for this purpose. In this review, we present the progress in NGS application for assessing mutagenicity of chemicals, including the methods used for detecting the induced mutations, bioinformatics tools for analyzing the sequencing data, and chemicals whose mutagenicity has been evaluated using NGS. Available information suggests that NGS technology has unparalleled advantages for evaluating mutagenicity of chemicals can be applied for the next generation of mutagenicity tests.

中文翻译：

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使用下一代测序评估化学诱变性：综述。

突变是DNA核苷酸序列中的可遗传变化，可能导致许多不利影响。遗传毒性测定已用于鉴定化学诱变性。最近，下一代测序（NGS）已用于此目的。在本综述中，我们介绍了NGS在评估化学品诱变性方面的应用进展，包括用于检测诱导突变的方法，用于分析测序数据的生物信息学工具以及已使用NGS评估了诱变性的化学品。现有信息表明，NGS技术在评估化学物质的致突变性方面具有无与伦比的优势，可用于下一代致突变性测试。