Numerous pyrrolizidine alkaloid (PA) poisoning cases have been documented worldwide. Protein covalent binding with reactive metabolites generated from metabolic activation of PAs to form pyrrole-protein adducts is suggested to be a primary mechanism of PA-induced toxicities. The present study aimed to develop antibodies for diagnosis of PA exposure. Polyclonal antibodies were raised in rabbits and proven to specifically recognize pyrrole-protein adducts regardless of amino acid residues modified by the reactive metabolites of PAs. The developed antibodies were successfully applied to detect pyrrole-protein adducts in blood samples obtained from PA-treated rats and exhibited a potential for the clinical diagnosis of PA exposure.

中文翻译：

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免疫测定方法可诊断出吡咯烷核生物碱的暴露。

世界范围内已记录了许多吡咯烷核生物碱（PA）中毒病例。蛋白质与PA的代谢活化产生的反应性代谢物共价结合形成吡咯-蛋白质加合物被认为是PA诱导毒性的主要机制。本研究旨在开发用于诊断PA暴露的抗体。多克隆抗体可在兔中产生，并被证明可以特异性识别吡咯蛋白加合物，而不受PAs反应性代谢物修饰的氨基酸残基的影响。所开发的抗体已成功地应用于检测从PA治疗的大鼠获得的血液样本中的吡咯蛋白加合物，并具有临床诊断PA暴露的潜力。