Unicellular model cyanobacterium Synechocystis sp. PCC 6803 has received considerable attention as a sustainable energy resource because of its photosynthetic machinery. However, two-component signal transduction systems (TCSTSs) in regulating central carbohydrate and energy metabolism of cyanobacteria are still poorly understood due to their diversity and functional complication. In this study, by comparing the growth of knockout mutants of 44 response regulators (RRs) of TCSTSs in Synechocystis, several RR mutants demonstrating differential growth patterns were identified under auto- or photomixotrophic conditions. However, in spite of no growth difference observed for the remaining RR mutants, liquid chromatography-mass spectrometry based metabolomic profile analysis showed that a widespread crosstalk of TCSTSs in regulating central carbohydrate and energy metabolism of Synechocystis was identified, while most of them showed diverse patterns during different trophic types or growth stages. Furthermore, an integrative analysis between evolutionary relationships and metabolomic profiles revealed some pairs of paralogous RRs with highly functional convergence, suggesting the possible conserved functions of Synechocystis TCSTSs during evolution. This study laid an important basis for understanding the function of TCSTSs in photosynthetic cyanobacteria.

中文翻译：

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两组分信号转导系统在串养藻自养和光合营养生长过程中调节中心碳水化合物和能量代谢的串扰。PCC 6803。

单细胞模型蓝藻Synechocystis sp。PCC 6803由于具有光合作用机制，已作为可持续能源受到了相当大的关注。然而，由于其多样性和功能复杂性，用于调节蓝藻的中心碳水化合物和能量代谢的两组分信号转导系统（TCSTS）仍然知之甚少。在这项研究中，通过比较突囊藻中TCSTS的44个应答调节因子（RR）的敲除突变体的生长，在自养或光合营养条件下，鉴定了几种具有不同生长模式的RR突变体。但是，尽管未观察到其余RR突变体的生长差异，基于液相色谱-质谱的代谢组学特征分析表明，TCSTS在调节集胞藻的中央碳水化合物和能量代谢中存在广泛的串扰，而大多数在不同的营养类型或生长阶段表现出不同的模式。此外，在进化关系和代谢组学谱之间的综合分析揭示了几对具有高功能收敛性的旁系RR，这表明突囊藻TCSTS在进化过程中可能具有保守的功能。该研究为了解TCSTSs在光合作用蓝细菌中的功能奠定了重要基础。进化关系和代谢组学谱之间的综合分析揭示了几对具有高功能收敛性的旁系同源受体，这表明突囊藻TCSTS在进化过程中可能具有保守的功能。该研究为了解TCSTSs在光合作用蓝细菌中的功能奠定了重要基础。进化关系和代谢组学谱之间的综合分析揭示了几对具有高功能收敛性的旁系同源受体，这表明突囊藻TCSTS在进化过程中可能具有保守的功能。该研究为理解TCSTSs在光合作用蓝细菌中的功能奠定了重要基础。