Translationally controlled tumor protein (TCTP) is a cytosolic protein with microtubule stabilization and calcium-binding activities. TCTP is expressed in most organs including the nervous system. However, detailed distribution and functional significance of TCTP in the brain remain unexplored. In this study, we investigated the global and subcellular distributions of TCTP in the mouse brain. Immunohistochemical analyses with anti-TCTP revealed that TCTP was widely distributed in almost all regions of the brain including the cerebral cortex, thalamus, hypothalamus, hippocampus, and amygdala, wherein it was localized in axon tracts and axon terminals. In the hippocampus, TCTP was prominently localized to axon terminals of the perforant path in the dentate gyrus, the mossy fibers in the cornu ammonis (CA)3 region, and the Schaffer collaterals in the CA1 field, but not in cell bodies of granule cells and pyramidal neurons, and in their dendritic processes. Widespread distribution of TCTP in axon tracts and axon terminals throughout the brain suggests that TCTP is likely involved in neurotransmitter release and/or maintaining synaptic structures in the brain, and that it might have a role in maintaining synaptic functions and synaptic configurations important for normal cognitive, stress and emotional functions.

中文翻译：

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小鼠海马神经元轴突末端翻译控制肿瘤蛋白的免疫组织化学定位。

翻译控制的肿瘤蛋白（TCTP）是具有微管稳定和钙结合活性的胞质蛋白。TCTP在包括神经系统在内的大多数器官中都有表达。然而，TCTP在大脑中的详细分布和功能意义尚待探索。在这项研究中，我们调查了小鼠脑中TCTP的整体和亚细胞分布。用抗TCTP进行的免疫组织化学分析显示，TCTP广泛分布在大脑的几乎所有区域，包括大脑皮层，丘脑，下丘脑，海马和杏仁核，其中TCTP定位于轴突道和轴突末端。在海马中，TCTP主要位于齿状回穿孔路径的轴突末端，即角膜氨化（CA）3区的苔藓纤维，Schaffer的侧支在CA1区域，但不在颗粒细胞和锥体神经元的细胞体中以及在其树突状过程中。TCTP在整个大脑的轴突区域和轴突末端广泛分布，这表明TCTP可能参与神经递质的释放和/或维持大脑中的突触结构，并且它可能在维持对于正常认知重要的突触功能和突触构型中发挥作用，压力和情绪功能。