GPR35, a family A orphan G protein-coupled receptor, has been implicated in inflammatory, neurological, and cardiovascular diseases. However, not much is known about the signaling and functions of GPR35. We performed a label-free kinome short hairpin RNA screen and identified a putative signaling network of GPR35 in HT-29 cells, some of which was validated using gene expression, biochemical and cellular assays. The results showed that GPR35 induced hypoxia-inducible factor 1α, and was involved in synaptic transmission, sensory perception, the immune system, and morphogenetic processes. Collectively, our data suggest that GPR35 may play an important role in response to hypoxic stress and be a potential target for the treatment of inflammatory, cardiovascular, and neurological disorders.

中文翻译：

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GPR35信号传导的化学基因组分析。

GPR35是一种家族A孤儿G蛋白偶联受体，与炎症，神经系统疾病和心血管疾病有关。但是，关于GPR35的信号传导和功能知之甚少。我们进行了无标记的kinome短发夹RNA筛选，并鉴定了HT-29细胞中GPR35的假定信号网络，其中一些已通过基因表达，生化和细胞分析验证。结果表明，GPR35诱导了缺氧诱导因子1α，并参与突触传递，感觉知觉，免疫系统和形态发生过程。总体而言，我们的数据表明，GPR35在低氧应激反应中可能起重要作用，并且可能是治疗炎症，心血管和神经系统疾病的潜在靶标。