BACKGROUND Insular function has gradually become a topic of intense study in cognitive research. Recognition memory is a commonly studied type of memory in memory research. GABABR has been shown to be closely related to memory formation. In the present study, we used intellicage, which is a new intelligent behavioural test system, and a bilateral drug microinjection technique to inject into the bilateral insula, to examine the relationship between GABABR and recognition memory. METHODS Male Sprague-Dawley rats were randomly divided into control, Sham, Nacl, baclofen and CGP35348 groups. Different testing procedures were employed using intellicage to detect changes in rat recognition memory. The expression of GABABR (GB1, GB2) in the insula of rats was determined by immunofluorescence and western blotting at the protein level. In addition, the expression of GABABR (GB1, GB2) was detected by RT-PCR at the mRNA level. RESULTS The results of the intellicage test showed that recognition memory was impaired in terms of position learning, punitive learning and punitive reversal learning by using baclofen and CGP35348. In position reversal learning, no significant differences were found in terms of cognitive memory ability between the control groups and the CGP and baclofen groups. Immunofluorescence data showed GABABR (GB1, GB2) expression in the insula, while data from RT-PCR and western blot analysis demonstrated that the relative expression of GB1 and GB2 was significantly increased in the baclofen group compared with the control groups. In the CGP35348 group, the expression of GB1 and GB2 was significantly decreased, but there was no significant difference in GB1 or GB2 expression in the control groups. CONCLUSIONS GABABR expression in the insula plays an important role in the formation of recognition memory in rats.

中文翻译：

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用智能体观察到的岛突中GABA B受体对识别记忆的影响。

背景技术岛突功能已逐渐成为认知研究中深入研究的主题。识别记忆是记忆研究中一种常用的记忆类型。GABABR已被证明与记忆形成密切相关。在本研究中，我们使用了智能化的智能行为测试系统，以及双侧药物注射技术，以检测GABABR与识别记忆之间的关系。方法将雄性Sprague-Dawley大鼠随机分为对照组，Sham，Nacl，baclofen和CGP35348组。使用智能技术采用不同的测试程序来检测大鼠识别记忆的变化。通过在蛋白质水平上的免疫荧光和蛋白质印迹测定大鼠肠岛中GABABR（GB1，GB2）的表达。此外，RT-PCR检测mRNA水平的GABABR（GB1，GB2）表达。结果智力测试的结果表明，使用巴氯芬和CGP35348在位置学习，惩罚性学习和惩罚性逆转学习方面损害了识别记忆。在位置逆转学习中，对照组与CGP组和巴氯芬组之间在认知记忆能力方面没有发现显着差异。免疫荧光数据显示在岛中GABABR（GB1，GB2）表达，而RT-PCR和Western blot分析数据表明，与对照组相比，巴氯芬组中GB1和GB2的相对表达显着增加。在CGP35348组中，GB1和GB2的表达明显降低，但对照组的GB1或GB2表达没有明显差异。结论岛突中的GABABR表达在大鼠识别记忆的形成中起重要作用。