CD3+ T lymphocytes were isolated by positive magnetic separation from the peripheral blood of healthy donors. In the absence of any additional activating stimuli, interleukin-7 (IL-7) was shown to augment the levels of T cells expressing CD25 activation marker both in CD4-positive and in CD4-negative effector memory (CD45RA^-CD197^-) T cell subsets, as well as in terminally differentiated (CD45RA⁺CD197^-) T cells, without significantly affecting the activation status of naive (CD45RA⁺CD197⁺) and central memory (CD45RA^-CD197⁺) T cells. In addition, IL-7 noticeably enhanced the production of IL-2, interferon-γ (IFN-γ), and IL-10, but not IL-4, in T cells. The direct effects of IL-7 on T cell activation induced in vitro by MACSiBead™ particles coated with CD2, CD3, and CD28 antibodies (Abs) were also investigated. Upon cell activation, IL-7 significantly augmented the levels of CD25+ T cells in naive (CD45RA+CD197+), central memory (CD45RA^-CD197⁺), and effector memory (CD45RA^-CD197^-) T-cell compartments. In addition, IL-7 facilitated activation of CD4^- (but not CD4⁺) terminally differentiated effector (CD45RA⁺CD197^-) T cells. Finally, IL-7 was found to upregulate the production of IL-2, IFN-γ, IL-4, and IL-10 by activated T cells. In conclusion, we speculate that IL-7 is capable of enhancing functional T cell activity without causing significant functional inbalance between various T cell subsets.

中文翻译：

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白细胞介素7在体外对人T细胞功能的直接影响。

通过正磁分离从健康供体的外周血中分离出CD3 + T淋巴细胞。在不存在任何额外的活化刺激的，白介素-7（IL-7）显示出，以增加T细胞的表达CD25活化标记物都在CD4阳性和CD4阴性效应记忆（CD45RA水平^- CD197 ^-）T细胞的子集，以及在终末分化（CD45RA ⁺ CD197 ^-）T细胞，而不会影响显著幼稚（CD45RA的激活状态⁺ CD197 ⁺）和中枢记忆（CD45RA ^- CD197 ⁺）T细胞。此外，IL-7明显增强了T细胞中IL-2，干扰素-γ（IFN-γ）和IL-10的产生，但没有增强IL-4的产生。还研究了IL-7对由涂有CD2，CD3和CD28抗体（Abs）的MACSiBead™颗粒体外诱导的T细胞活化的直接作用。一旦细胞活化，IL-7显著增强CD25 + T细胞的水平在幼稚（CD45RA + CD197 +），中枢记忆（CD45RA ^- CD197 ⁺）和效应记忆（CD45RA ^- CD197 ^-）T细胞隔室。此外，IL-7促进CD4的活化^- （但不是CD4 ⁺）终末分化的效应（CD45RA ⁺ CD197 ^-）T细胞。最后，发现IL-7通过活化的T细胞上调IL-2，IFN-γ，IL-4和IL-10的产生。总之，我们推测IL-7能够增强功能性T细胞活性，而不会引起各个T细胞亚群之间明显的功能失衡。