Partial resistance quantitative trait loci (QTLs) Rphq11 and rphq16 against Puccinia hordei isolate 1.2.1 were previously mapped in seedlings of the mapping populations Steptoe/Morex and Oregon Wolfe Barleys, respectively. In this study, QTL mapping was performed at adult plant stage for the two mapping populations challenged with the same rust isolate. The results suggest that Rphq11 and rphq16 are effective only at seedling stage, and not at adult plant stage. The cloning of several genes responsible for partial resistance of barley to P. hordei will allow elucidation of the molecular basis of this type of plant defence. A map-based cloning approach requires to fine-map the QTL in a narrow genetic window. In this study, Rphq11 and rphq16 were fine-mapped using an approach aiming at speeding up the development of plant material and simplifying its evaluation. The plant materials for fine-mapping were identified from early plant materials developed to produce QTL-NILs. The material was first selected to carry the targeted QTL in heterozygous condition and susceptibility alleles at other resistance QTLs in homozygous condition. This strategy took four to five generations to obtain fixed QTL recombinants (i.e., homozygous resistant at the Rphq11 or rphq16 QTL alleles, homozygous susceptible at the non-targeted QTL alleles). In less than 2 years, Rphq11 was fine-mapped into a 0.2-cM genetic interval and a 1.4-cM genetic interval for rphq16. The strongest candidate gene for Rphq11 is a phospholipid hydroperoxide glutathione peroxidase. Thus far, no candidate gene was identified for rphq16.

中文翻译：

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大麦对叶锈病的植物阶段特异性部分抗性相关基因的高分辨率作图。

先前已将分别对制图种群Steptoe / Morex和俄勒冈州Wolfe Barleys的幼苗绘制了针对大麦楠（Puccinia hordei）分离株1.2.1的部分抗性定量性状基因座（QTL）Rphq11和rphq16。在这项研究中，在成年植株阶段对两个受相同锈病分离菌侵害的作图群体进行了QTL作图。结果表明，Rphq11和rphq16仅在幼苗阶段有效，而在成年植物阶段无效。克隆导致大麦对大果疟原虫部分抗性的几种基因将阐明这种植物防御的分子基础。基于图的克隆方法需要在狭窄的遗传窗口中精确定位QTL。在这项研究中，Rphq11和rphq16使用旨在加快植物材料开发和简化其评估的方法进行了精细映射。从开发用于生产QTL-NIL的早期植物材料中鉴定出用于精细映射的植物材料。首先选择该材料在杂合条件下携带目标QTL，在纯合条件下携带其他抗性QTL的易感等位基因。该策略花费了四到五代的时间来获得固定的QTL重组体（即，在Rphq11或rphq16 QTL等位基因上是纯合的，在非靶向QTL等位基因上是纯合的）。在不到2年的时间里，Rphq11被精细映射为rphq16的0.2-cM遗传间隔和1.4-cM的遗传间隔。Rphq11最强的候选基因是磷脂氢过氧化物谷胱甘肽过氧化物酶。迄今，