Objectives: To investigate the effects of sodium ascorbate (SA) (5–3125 μM) and a combination of SA and Trolox (25 and 125 μM) on oxidative changes generated in red blood cells (RBCs) followed by up to 20 days refrigerated storage.

Methods: RBCs were isolated from CPD-preserved human blood. Percentage of hemolysis and extracellular activity of lactate dehydrogenase (LDH) were measured to assess the RBC membrane integrity. Lipid peroxidation (LPO), glutathione (GSH) and total antioxidant capacity (TAC) were quantified by thiobarbituric acid-reactive substances, Ellman’s reagent and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate) -based assay, respectively.

Results: SA failed to reduce the storage-induced hemolysis and RBC membrane permeability. Addition of SA resulted in a concentration-independent LPO inhibition and increased TAC. A combination of SA/Trolox supplemented to the RBC medium significantly inhibited hemolysis, LDH leakage, LPO, GSH depletion and enhanced TAC.

Discussion: The effects of vitamin C action are closely concentration-dependent and may be modulated by a variety of compounds (e.g. Hb degradation products) released from RBCs during the prolonged storage, changing its properties from anti- to pro-oxidative. The two different class antioxidants (SA/Trolox) could possibly cooperate to be good potential RBC storage additives ensuring both antiradical and membrane stabilizing protection.

目的：研究抗坏血酸钠（SA）（5–3125μM）以及SA和Trolox的组合（25和125μM）对红细胞（RBC）产生的氧化变化的影响，然后冷藏保存长达20天。

方法：从保存了CPD的人血中分离出RBC。测量溶血百分比和乳酸脱氢酶（LDH）的细胞外活性，以评估RBC膜的完整性。脂质过氧化（LPO），谷胱甘肽（GSH）和总抗氧化能力（TAC）通过硫代巴比妥酸反应性物质，Ellman试剂和2,2'-叠氮基双-（3-乙基苯并噻唑啉-6-磺酸盐）进行定量基于基础的分析。

结果： SA未能降低储存诱导的溶血和RBC膜通透性。添加SA导致浓度依赖性LPO抑制和TAC增加。补充RBC培养基的SA / Trolox组合可显着抑制溶血，LDH渗漏，LPO，GSH耗竭并增强TAC。

讨论：维生素C的作用与浓度密切相关，在长期储存过程中，红细胞释放出的多种化合物（例如Hb降解产物）可能会调节维生素C的作用，从而将其性能从抗氧化性转变为促氧化性。两种不同类型的抗氧化剂（SA / Trolox）可能会协同作用，成为潜在的RBC储存添加剂，从而确保抗自由基和膜稳定的保护。