Objectives: The study was conducted to assess the redox status of Drosophila flies upon oral intake of insulin-mimetic salt, sodium molybdate (Na₂MoO₄).

Methods: Oxidative stress parameters and activities of antioxidant and associated enzymes were analyzed in two-day-old D. melanogaster insects after exposure of larvae and newly eclosed adults to three molybdate levels (0.025, 0.5, or 10 mM) in the food.

Results: Molybdate increased content of low molecular mass thiols and activities of catalase, superoxide dismutase, glutathione-S-transferase, and glucose-6-phosphate dehydrogenase in males. The activities of these enzymes were not affected in females. Males exposed to molybdate demonstrated lower carbonyl protein levels than the control cohort, whereas females at the same conditions had higher carbonyl protein content and catalase activity than ones in the control cohort. The exposure to 10 mM sodium molybdate decreased the content of protein thiols in adult flies of both sexes. Sodium molybdate did not affect the activities of NADP-dependent malate dehydrogenase and thioredoxin reductase in males or NADP-dependent isocitrate dehydrogenase in either sex at any concentration.

Discussion: Enhanced antioxidant capacity in upon Drosophila flies low molybdate levels in the food suggests that molybdate can be potentially useful for the treatment of certain pathologies associated with oxidative stress.

目的：本研究旨在评估果蝇口服胰岛素模拟盐钼酸钠 (Na ₂ MoO ₄ ) 后的氧化还原状态。

方法：将两天大的黑腹果蝇幼虫和新孵出的成虫暴露于食物中的三种钼酸盐水平（0.025、0.5 或 10 mM）后，分析氧化应激参数以及抗氧化剂和相关酶的活性。

结果：钼酸盐增加了雄性体内低分子量硫醇的含量以及过氧化氢酶、超氧化物歧化酶、谷胱甘肽-S-转移酶和葡萄糖-6-磷酸脱氢酶的活性。这些酶的活性在女性中不受影响。暴露于钼酸盐的雄性表现出比对照组更低的羰基蛋白水平，而相同条件下的雌性比对照组具有更高的羰基蛋白含量和过氧化氢酶活性。暴露于 10 mM 钼酸钠会降低雌雄成年果蝇的蛋白质硫醇含量。任何浓度的钼酸钠都不影响雄性中NADP依赖性苹果酸脱氢酶和硫氧还蛋白还原酶的活性，也不影响任何性别中NADP依赖性异柠檬酸脱氢酶的活性。

讨论：果蝇食物中钼酸盐含量低，抗氧化能力增强，表明钼酸盐可能可用于治疗与氧化应激相关的某些病理。