The expression pattern and biological role of long non-coding RNA (lncRNA) in cancer has been reported to be involved in many cancers. Here, we report the expression and biological role of a newly discovered lncRNA NmrA-like family domain containing 1 pseudogene (Loc344887) in gallbladder cancer (GBC). In this study, we found that the expression of Loc344887 was significantly elevated in GBC tissues and cell lines when compared with matched normal tissues and normal epithelial bile duct cell line, respectively. High Loc344887 was associated with larger tumor size. Loc344887 was upregulated significantly after ectopic expression of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) in GBC cells. Downregulation of Loc344887 in GBC cells suppressed cell proliferation, blocked cells in G0/S phase, and decreased the migration and invasion cell numbers. In addition, downregulation of Loc344887 decreased the expression of transcription factor Twist, mesenchymal marker Vimentin, and N-cadherin and increased the expression of epithelial maker E-cadherin, which could prompt a mesenchymal-to-epithelial transition phenotype. These results demonstrated that Loc344887 might contribute to cell proliferation and epithelial-to-mesenchymal transition process in GBC, which might be a potential therapeutic target.

中文翻译：

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含有1个假基因Loc344887的NmrA样家族结构域在胆囊癌中被扩增并促进上皮-间质转化。

据报道，长非编码RNA（lncRNA）在癌症中的表达模式和生物学作用与许多癌症有关。在这里，我们报告胆囊癌（GBC）中新发现的包含1个假基因（Loc344887）的lncRNA NmrA样家族结构域的表达和生物学作用。在这项研究中，我们发现与匹配的正常组织和正常上皮胆管细胞系相比，Loc344887在GBC组织和细胞系中的表达显着升高。高Loc344887与更大的肿瘤大小有关。在GBC细胞中异位表达核因子（类胡萝卜素衍生的2）样2（Nrf2）后，Loc344887显着上调。GBC细胞中Loc344887的下调抑制了细胞增殖，阻止了G0 / S期的细胞，并减少了迁移和侵袭细胞的数量。此外，Loc344887的下调降低了转录因子Twist，间充质标志物波形蛋白和N-钙黏着蛋白的表达，并增加了上皮制造商E-钙黏着蛋白的表达，这可能提示了从间质到上皮的转变表型。这些结果表明Loc344887可能有助于GBC中的细胞增殖和上皮-间质转化过程，这可能是潜在的治疗靶标。