Background

In order to detect the antimicrobial mechanism of combined treatment of cinnamon oil and gamma irradiation (GI), the membrane fatty acids and proteins characteristics of Shewanella putrefaciens (S. putrefaciens) treated with cinnamon oil and GI, and the distribution of cinnamon oil in S. putrefaciens were observed in this study.

Results

The membrane lipid profile of S. putrefaciens was notably damaged by treatments of cinnamon oil and the combination of cinnamon oil and GI, with significantly fatty acids decrease in C14:0, C16:0, C16:1, C17:1, C18:1 (p < 0.05). The SDS-PAGE result showed that GI did not have obvious effect on membrane proteins (MP), but GI combined with cinnamon oil changed the MP subunits. Cinnamaldehyde, the main component of cinnamon oil, can not transport into S. putrefaciens obviously. It was transformed into cinnamyl alcohol in the nutrient broth with the action of S. putrefaciens. This indicated that the antimicrobial action of cinnamon oil mainly happened on the membrane of S. putrefaciens.

Conclusion

Cinnamon oil could act on the membrane of S. putrefaciens with the damage of fatty acids and proteins, and GI would increase the destructive capability of cinnamon oil on the membrane fatty acids and proteins of S. putrefaciens.

中文翻译：

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肉桂油和γ射线辐照处理的腐烂希瓦氏菌膜脂肪酸和蛋白质的变化。

背景

为了检测肉桂油和伽马射线（GI）联合处理的抗菌机制，肉桂油和GI处理的希瓦氏假单胞菌（S. putrefaciens）的膜脂肪酸和蛋白质特性，以及肉桂油在S中的分布在这项研究中观察到腐烂。

结果

肉桂油和肉桂油与GI的组合处理可显着破坏腐烂链球菌的膜脂谱，其中C14：0，C16：0，C16：1，C17：1，C18：1中的脂肪酸显着降低（p  <0.05）。SDS-PAGE结果表明，GI对膜蛋白（MP）没有明显影响，但GI与肉桂油联合可改变MP亚基。肉桂油的主要成分肉桂醛显然不能转运到腐烂链球菌中。在腐乳链球菌的作用下，将其在营养肉汤中转化为肉桂醇。这表明肉桂油的抗菌作用主要发生在腐烂链球菌的膜上。

结论

肉桂油可以通过脂肪酸和蛋白质的破坏作用于腐烂链球菌的膜上，而胃肠道将增加肉桂油对腐烂链球菌的膜脂肪酸和蛋白质的破坏能力。