The present study was conducted to evaluate the feasibility of enzymatic hydrolysis of carotenoid esters from Tagetes erecta using lipases from the yeast of Yarrowia lipolytica, with the aim of obtaining free lutein. The optimal concentrations of seven nutrients, considering the production of lipases relative to biomass (Yp/x) as the response variable, were determined in flask fermentations. In addition, we studied the effect on hydrolysis of growing Y. lipolytica in the presence of the oleoresin of the marigold flower in flask and stirred tank. Furthermore, hydrolysis of the oleoresin using the lipases from this microorganism was compared with the hydrolysis using lipases from Rhizopus oryzae. Cultured in the presence of marigold oleoresin, Y. lipolytica showed an increase in free carotenoids of 12.41% in flask and 8.8% in stirred tank, representing a fourfold and a threefold increase compared to the initial value in the fermentation, respectively. When lipases from the supernatant from both microorganisms were used for only 14 h hydrolysis experiments, a slight increase was achieved compared to a blank. We concluded that carotenoid esters of the oleoresin could not be completely hydrolyzed in 14 h by these lipases, but that growing Y. lipolytica in the presence of marigold oleoresin gives until fourfold production of free carotenoids in 72 h fermentations.

中文翻译：

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通过解脂耶氏酵母脂肪酶的作用，水解万寿菊中的类胡萝卜素酯。


本研究旨在评估使用解脂耶氏酵母脂肪酶酶解万寿菊类胡萝卜素酯的可行性，以获得游离叶黄素。考虑到脂肪酶的产量相对于生物量 (Yp/x) 作为响应变量，在烧瓶发酵中确定了七种营养素的最佳浓度。此外，我们研究了在烧瓶和搅拌罐中万寿菊花油树脂存在下对正在生长的解脂耶氏酵母水解的影响。此外，将使用来自该微生物的脂肪酶的油树脂水解与使用来自米根霉的脂肪酶的水解进行比较。在万寿菊油树脂存在下培养，解脂耶氏酵母的游离类胡萝卜素在烧瓶中增加了 12.41%，在搅拌罐中增加了 8.8%，与发酵初始值相比分别增加了四倍和三倍。当两种微生物上清液中的脂肪酶仅用于 14 小时水解实验时，与空白相比，实现了轻微的增加。我们得出的结论是，油树脂的类胡萝卜素酯不能在 14 小时内被这些脂肪酶完全水解，但在万寿菊油树脂存在下生长的解脂耶氏酵母在 72 小时的发酵中直至产生四倍的游离类胡萝卜素。