This study aims to understand and better control the main biological mechanisms and parameters modulating the various phenomena affecting Yarrowia lipolytica JMY775 and its lipids accumulation. The results obtained in this study stress forward that the use of an original tool, consisting of coupling bioreactors to online flow cytometry, is highly efficient. Throughout 48 h of culturing, this emerging process allowed an online continuous observation of the effects of pH and/or aeration on the cell growth and dimorphism and lipid accumulation by Y. lipolytica. This present study showed clearly that online flow cytometry is an advantageous tool for the real-time monitoring of microbial culture at a single-cell level. Indeed, the present investigation showed for the first time that profiling of the various phenomena and their monitoring upon culture time is now possible by coupling online cytometry with culture bioreactors.

中文翻译：

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在线流式细胞仪是一个有趣的研究过程，用于监测油脂酵母酵母解脂耶氏酵母JMY 775中的脂质积累，二态性和细胞生长。

本研究旨在了解和更好地控制调节解脂耶氏酵母JMY775及其脂质积累的各种现象的主要生物学机制和参数。这项研究中获得的结果强调，使用原始工具（包括将生物反应器与在线流式细胞仪偶联）是非常有效的。在整个培养的48小时中，这种新兴过程可以在线连续观察pH和/或通气对解脂耶氏酵母的细胞生长，二态性和脂质积累的影响。本研究清楚地表明，在线流式细胞仪是一种在单细胞水平上实时监测微生物培养的有利工具。的确，