BACKGROUND Olfaction is often affected in parkinsonian patients, but dopaminergic cells in the olfactory bulb are not affected by some Parkinson-inducing drugs. We investigated whether the drug MPTP produces the olfactory deficits typical of Parkinson and affects the olfactory bulb in mice. FINDINGS Lesioned and control mice were tested for olfactory search, for motor and exploratory behavior. Brains and olfactory mucosa were investigated via immunohistochemistry for thyrosine hydroxylase, Olfactory Marker Protein and cyclic AMP-dependent protein kinase as an intracellular pathway involved in dopaminergic neurotransmission. MPTP induced motor impairment, but no deficit in olfactory search. Thyrosine hydroxylase did not differ in olfactory bulb, while a strong decrease was detected in substantia nigra and tegmentum of MPTP mice. Olfactory Marker Protein decreased in the olfactory bulb of MPTP mice, while a cyclic AMP-dependent protein kinase increased in the inner granular layer of MPTP mice. CONCLUSIONS MPTP mice do not present behavioural deficits in olfactory search, yet immunoreactivity reveals modifications in the olfactory bulb, and suggests changes in intracellular signal processing, possibly linked to neuron survival after MPTP.

中文翻译：

---

药物诱发的帕金森氏病可调节小鼠嗅球中的蛋白激酶A和嗅觉标记蛋白。

背景技术嗅觉在帕金森氏病患者中通常受到影响，但是嗅球中的多巴胺能细胞不受某些帕金森氏诱导药物的影响。我们调查了药物MPTP是否会产生帕金森典型的嗅觉缺陷并影响小鼠的嗅球。结果对患病小鼠和对照小鼠进行嗅觉搜索，运动和探索行为的测试。通过免疫组化研究了大脑和嗅觉粘膜的酪氨酸羟化酶，嗅觉标记蛋白和环状AMP依赖性蛋白激酶，这是一种参与多巴胺能神经传递的细胞内途径。MPTP引起运动障碍，但嗅觉搜索无缺陷。酪氨酸羟化酶在嗅球中没有差异，而在MPTP小鼠的黑质和盖膜中检测到强烈的降低。嗅觉标记蛋白在MPTP小鼠的嗅球中减少，而环状AMP依赖的蛋白激酶在MPTP小鼠的内颗粒层中增加。结论MPTP小鼠在嗅觉搜索中并未表现出行为缺陷，但免疫反应揭示了嗅球的修饰，并提示细胞内信号处理的变化，可能与MPTP术后的神经元存活有关。