Subclinical hypocalcaemia (SH) is an important metabolic disease in dairy cows that has a serious impact on production performance. The objective of this study was to investigate novel aspects of pathogenesis using proteomics technology to identify proteins that are differentially expressed in diseased and healthy animals. Dairy cows were divided into an SH group (T, n = 10) and a control group (C, n = 10) based on plasma calcium concentration. A total of 398 differentially expressed proteins were identified, of which 265 proteins were overlapped in the two parallel experiments. Of these, 24 differentially expressed proteins were statistically significant. Gene Ontology analysis yielded 74 annotations, including 7 cellular component, 55 biological process and 12 molecular function categories. Bioinformatics analysis indicated that calcium regulation, immune and inflammatory response, blood coagulation and complement pathway were all related to SH. Our iTRAQ/LC-MS/MS (isobaric tags for relative and absolute quantification/liquid chromatography-mass spectrometry/mass spectrometry) approach proved highly effective for plasma protein profiling of dairy cows with SH, and the results pave the way for further studies in this area.

中文翻译：

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亚临床低钙血症奶牛血浆蛋白的蛋白质分析。

亚临床低钙血症（SH）是奶牛重要的代谢性疾病，严重影响生产性能。本研究的目的是利用蛋白质组学技术来研究发病机制的新方面，以识别在患病和健康动物中差异表达的蛋白质。根据血浆钙浓度将奶牛分为 SH 组（T，n = 10）和对照组（C，n = 10）。共鉴定出398个差异表达蛋白，其中265个蛋白在两个平行实验中重叠。其中，24个差异表达蛋白具有统计学意义。基因本体分析产生了 74 个注释，包括 7 个细胞成分、55 个生物过程和 12 个分子功能类别。生物信息学分析表明，钙调节、免疫和炎症反应、凝血和补体途径均与SH有关。事实证明，我们的 iTRAQ/LC-MS/MS（用于相对和绝对定量的同量异位标签/液相色谱-质谱/质谱）方法对于 SH 奶牛的血浆蛋白分析非常有效，结果为进一步研究铺平了道路。这片区域。