Search for naturally grown food has stimulated the biotechnological production of carotenoids. Therefore, the use of the yeast Xanthophyllomonas dendrorhous has been researched due to its abilities to assimilate different sources as substrates and to produce high amounts of carotenoids. Furthermore, alternative sources have been used as the culture medium to reduce costs and environmental impact. A potent carotenoid is astaxanthin in view of its health-promoting and antioxidative properties. It consists of different geometrical isomers with trans and cis configuration. In X. dendrorhous this carotenoid is mostly found in the trans form, but cis isomers can also be found. Carotenoid production was investigated in culture medium containing by-products such as raw glycerol (from biodiesel) and parboiled rice effluent. The effects of the culture medium components on biomass concentration and specific and volumetric productions of carotenoids were verified by the Plackett-Burman design. Cultivations were carried out with yeast Xanthophyllomonas dendrorhous NRRL Y-17268 at 25 °C and 150 rpm for 168 h. In this study, maximum production of carotenoids was obtained under the following conditions (in g/L): raw glycerol 10, glucose 10, yeast extract 10, malt extract 10 and peptone 1 at pH=6. Resulting specific and volumetric productions of carotenoids were 326.8 and 4.1 µg/g, respectively.

中文翻译：

---

用于生产类胡萝卜素的生甘油和半熟米废水：培养基组成和初始pH的影响。

寻求自然生长的食物刺激了类胡萝卜素的生物技术生产。因此，由于其具有吸收不同来源作为底物并产生大量类胡萝卜素的能力，因此已经研究了酵母黄单胞菌的使用。此外，替代来源已被用作培养基以降低成本和对环境的影响。考虑到其促进健康和抗氧化的特性，有效的类胡萝卜素是虾青素。它由不同的几何异构体的反式与顺式构型。在X. dendrorhous中，这种类胡萝卜素主要以反式形式存在，但是顺式异构体也可以找到。在含有副产物的培养基中研究了类胡萝卜素的产生，这些副产物包括未加工的甘油（来自生物柴油）和煮过的稻米出水。通过Plackett-Burman设计验证了培养基成分对生物质浓度以及类胡萝卜素的比重和体积产量的影响。用酵母黄单胞菌NRRL Y-17268在25℃和150rpm下培养168小时。在这项研究中，在以下条件下（以g / L为单位）获得了最大量的类胡萝卜素：原始甘油10，葡萄糖10，酵母提取物10，麦芽提取物10和蛋白ept 1，pH = 6。所产生的类胡萝卜素的比产量和体积产量分别为326.8和4.1 µg / g。